In Vitro Propagation of Alternanthera sessilis L.  from Internode Explant

Dimpy Das; P. K. Borua

doi:10.9734/BBJ/2014/6261

Research Article Open access CC BY 3.0

In Vitro Propagation of Alternanthera sessilis L. from Internode Explant

Dimpy Das, P. K. Borua

Biotechnology Journal International · pp. 74–80 · Published 9 Nov 2013

10.9734/BBJ/2014/6261

Abstract

An efficient protocol was developed for micropropagation of Alternanthera sessilis L. from internode explant. Callus was developed from the cut ends and surface of the internode explants when the Murashige and Skoog (MS) medium was supplemented with 0.5mg/L or 1.0mg/L 2,4-D. The greatest mean number of shoots (124) with the highest mean shoot length (9.3cm) was obtained when calli were cultured on the MS medium with 1.0mg/L each of 6-Benzylaminopurine and adenine sulfate. Early and maximum root induction was noted when shoots were cultured in half strength MS basal medium. In vitro regenerated plants were successfully hardened in a mixture of soil and cow dung in ratio of 3:1 and transferred to field. Cent percent plants survived in field condition, morphologically identical to parent plant and showed normal flowering.

Alternanthera sessilis medicinal plant micropropagation internode callus shoot bud induction

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Dimpy Das, P. K. Borua (2013). In Vitro Propagation of Alternanthera sessilis L. from Internode Explant. Biotechnology Journal International , 74–80. https://doi.org/10.9734/BBJ/2014/6261

Affiliations

Dimpy Das — M.C. College, Barpeta, Assam- 781 301, India
P. K. Borua — Department of Life Sciences, Dibrugarh University, Assam- 786 004, India

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