In vitro Membranous activity of Biosynthesized Gold Nanoparticle from Aqueous Leave Extract of Nelsonia canescens

Abstract

Nano drug delivery technologies have advantages of transporting the drug to target site to better enhance its effectiveness, bioavailability and reduces side effects with dose frequency. In this study, the reducing capability of Nelsonia canescens for gold nanoparticle synthesis and its membranous activity on MCF-7 cell have been investigated. The morphology, particle size and the functional group of the bio- reducing agent of the synthesized gold nanoparticle were investigated using High-Resolution Scanning Electron Microscopy (SEM), Zeta-sized nano and Fourier Transmission Infrared (FTIR) spectroscopy. The biosynthesis gold nanoparticle showed a strong surface plasmon resonance at 537.5 nm with an average size of 50nm. SEM image showed the morphology of the biosynthesized gold nanoparticle to be spherical with the possibility of aggregation. While FTIR confirms the reducing and capping agents of the synthesized gold Nanoparticle as hydroxyl group of alcohol or phenol with a strong signal at 3417.98 cm-1. The in vitro membranous activity shows concentration-dependent with an IC50 of 0.455 mg/l. Therefore, it could be concluded that Nelsonia canescens have the bioreductive capability to produce gold nanoparticle and in vitro membraneous activity on MCF-7 cell line.