Isolation and Screening for Biobutanol Producing Clostridium Species from the Soil Environment of Keffi Metropolis

Abstract

Clostridium species have been reported as one of the most common groups of bacteria that produce biobutanol. The Study was aimed at isolation of Clostridium species from the soil environment in Keffi Metropolis and screening for their ability to produce biobutanol. Ten different location soil samples were collected within keffi Metropolis. Nutrient Blood agar was used for the isolation of the Clostridium species. biobutanol production was carried out using Clostridial isolates and Lucas test was used to detect biobutanol production Clostridium isolates after fermentation. The results show that six (6) species of Clostridial were isolated with a percentage frequency of Clostridium difficile (30%), Clostridium botulinum (10%), Clostridium cylindrosporum (20%), Clostridium perfringens (10%), Clostridium acetobutylicum (20%) and Clostridium celerecrescens (10%). The result further revealed that all the six (6) isolates were found to be able to produce biobutanol. Biobutanol is an industrial organic chemical that can place fossil fuel if harnessed and production of Biobutanol using Clostridium in large quantity can be alternative to fossil fuel.