Investigation of Enzymes Binding to “Voglibose- an Antidiabetic Drug” and the Choice of Enzyme to be Used for Biosensing

Abstract

Aims: This paper mentions of the investigation of the enzymes binding to Voglibose and the choice of the enzyme which is suitable for drug enzyme interaction as used in biosensing. Voglibose is an antidiabetic drug which is reactive and hence used in very low concentrations. The existing methods of analysis of this drug are associated with certain disadvantages. Hence there is a need to establish alternative and simpler method of analysis which could be based on biosensing. The paper focuses on identification of the enzyme binding to the drug Voglibose. Study Design: In this work there are two enzyme namely alpha amylase and alpha glucosidase which are considered as the potential targets for drug enzyme interaction for the drug Voglibose. The study is based on two approaches. The first one is molecular docking process done to verify the inhibition activity of the enzyme by the drug and the next method is chemical analysis to confirm the results obtained in molecular docking. Place and Duration of Study: This experiment was carried out as a part of the project under Indian Nanotechnology User Program (INUP) at IITB, Mumbai. Methodology: Molecular docking is a simulation procedure which is used to confirm the inhibition activity of alpha amylase and alpha glucosidase by Voglibose and compare their inhibition activities so as to choose the suitable enzyme for further applications. Chemical analysis is done to re-confirm the same and chose the target enzyme for biosensing applications. Results: The docking experiments are done to show that Voglibose inhibits both alpha amylase and alpha glucosidase but a more stable complex is formed with alpha amylase and hence alpha amylase is used for the chemical analysis to reconfirm its inhibition by Voglibose. Thus, alpha amylase can be used as the target enzyme for drug enzyme interactions with Voglibose for further applications in biosensing. Conclusion: The present In-silico and In-vitro swot analysis indicates that alpha glucosidase and alpha amylase binds to Voglibose. The interaction outcome from molecular docking and chemical analysis suggests that in future one can consider alpha amylase as a choice of target enzyme in applications of biosensing.