Isolation, Bioactivity and Charaterisation of 3-Ethynyl-5-(2,3-dehydropyrrole) Pyridine from the Stem Bark of Adenanthera pavonina

Abstract

Natural products from medicinal plants either as pure compounds or standardized extracts, provide unlimited opportunities for new drug leads, because of the unmatched availability of chemical diversity. Due to an increasing demand for therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. The phytochemical screening was carried out via standard procedures while the isolation and characterization was done using different solvents via thin layer and column chromatography. The bioactivity studies of the purely active compound isolated was achieved using different clinical bacterial isolates, gram negative (E. coli and Salmonella typhi) and positive (Staphylococcus aureus); the radical scavenging power of the purely active compound was assayed using 2,2-diphenyl-1-picrylhydrazyl(DPPH) and characterization using GCMS, 1HNMR, 13CNMR and FTIR was carried out to facilitate structure elucidation. The focus of this paper is on the analytical and biological methodologies, which includes the extraction, isolation, bioactivity studies, and characterization of the purely active ingredients in the stem bark of Adenanthera pavonina.