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All published articles 904

Isolation and Identification of Actinomycetes from Mangrove Soil and Extraction of Secondary Metabolites for Antibacterial Activity

Anasuya Priyadarshini, Sameer Kumar Singdevsachan, Subodh Kumar Tripathy, Yugal Kishore Mohanta, Jayanta Kumar Patra & Bijay Kumar Sethi · Biotechnology Journal International · 2016

The mangrove ecosystem of India is an extensively unexplored source for actinomycetes with the potential to produce secondary metabolites of biological importance. In this study, twenty two actinomycetes were isolated from different soil samples collected from the Bhitarkanika ma...

Open access Research Article 10.9734/BBJ/2016/24102

Genetic Diversity of Native Bacillus thuringiensis Strains Isolated from Soil of Different Localities in Mali and their cry Gene Profile

R. Fané, D. Traoré, A. Kassogué, F. Samaké, A. H. Dicko, A. Hamadoun, F. H. Valicente & A. H. Babana · Biotechnology Journal International · 2016

Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Str...

Open access Research Article 10.9734/BBJ/2016/20432

Effect of Cytokinins on In vitro Propagation of Boucerosia umbellata (Haw.) Wight & Arn. (Syn.: Caralluma umbellata Haw.) from Nodal Explants of Field Grown Plants

B. Susheela, S. Sandhya Rani & T. Pullaiah · Biotechnology Journal International · 2016

An efficient protocol was described for the rapid in vitro multiplication of medicinal plant Boucerosia umbellata via enhanced axillary bud proliferation from nodal explants collected from young shoots of three-month-old field grown plants. Effects of cytokinins 6-benzylaminopuri...

Open access Research Article 10.9734/BBJ/2016/23676

Effects of Exogenous Salicylic Acid on the Antioxidative System in Bean Seedling Treated with Manganese

Issam Saidi, Nasreddine Yousfi, Wahbi Djebali & Yacine Chtourou · Biotechnology Journal International · 2016

In the present study we investigated the role of salicylic acid (SA) in regulating Mn-induced oxidative stress in bean (Phaseolus vulgaris) leaves. Exposure of plants to 100 µM Mn inhibited biomass production and intensively increased Mn accumulation in leaves. Concomitantly, Mn...

Open access Research Article 10.9734/BBJ/2015/15830

Frequency of Procedural Errors during Root Canal Treatment Performed by Interns

Syed Ajlal Akhtar, Fahad Ata Siddiqui, Abu Bakar Sheikh, Saqib Rashid, Zohaib Khurshid, Shariq Najeeb & Muhammad Sohail Zafar · Biotechnology Journal International · 2016

Aims: Chemo-mechanical preparation continues to be one of the most challenging steps in root canal treatment procedures. The aim of this study was to examine the frequency of procedural errors during root canal treatment performed by interns. Study Design: Cross sectional descrip...

Open access Research Article 10.9734/BBJ/2016/23768

Detection and Analysis of the Random Mutagenesis Site(s) of Xylanase Gene from Mutants of Bacillus subtilis subsp. spizizenii ATCC 6633

Hooi Ling Ho & Ajounmah Maryann Chinonso · Biotechnology Journal International · 2016

Aims: A total of five mutant strains of Bacillus subtilis subsp. spizizenii ATCC 6633 designated as the MXB 1, MXB 2, MXB 3, MXB 4 and MXB 5 were developed using random mutagenesis of ethyl methane sulfonate (EMS) and acridine orange (AO) in our previous study. Based on our prese...

Open access Research Article 10.9734/BBJ/2016/23057

Production of Bovine Herpesvirus-1 Vaccine Strains in MDBK Cells Using BelloCell Bioreactor

Lekshmi S. Rajan, Aman Kamboj, Bikash R. Prusty, Tapan Palai, L. R. Ananthakrishna, Mohini Saini & Praveen K. Gupta · Biotechnology Journal International · 2016

Aim: To produce bovine herpesvirus-1 (BoHV-1) vaccine strains in MDBK cells using an oscillating bioreactor, BelloCell. Place and Duration of Study: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India, between November 2013 and June...

Open access Research Article 10.9734/BBJ/2016/20401

Purification and Characterization of Pectin Methylesterase Produced in Solid State Fermentation by Aspergillus tubingensis

Mukesh Kumar Patidar, Anand Nighojkar, Sadhana Nighojkar & Anil Kumar · Biotechnology Journal International · 2016

Aim: Purification and characterization of pectin methylesterase produced by Aspergillus tubingensis in solid state fermentation. Study Design: Pectin methylesterase enzyme produced by A. tubingensis was extracted from the fermented solid medium and purified using chromatographic...

Open access Research Article 10.9734/BBJ/2016/23632

In vitro Direct Regeneration of Cotton (Gossypium spp.) Cultivars of Sudan

Iman K. A. Abdel Gadir, Marmar A. El Siddig, Hayfa H. A. Ibrahim & Adil A. El Hussein · Biotechnology Journal International · 2016

In vitro direct plantlets’ development from different explants of cotton was studied using nine cultivars which have been developed in Sudan. The influence of various phytohormones on morphogenetic response of different explants of each cultivar was tested. The best medium for mu...

Open access Research Article 10.9734/BBJ/2016/23109

The Effect of Drying and Salting on the Nutrient Composition and Organoleptic Properties of Talinum triangulare Leaves

Fred Omon Joseph Oboh & Gift Ejehiokhin Madojemu · Biotechnology Journal International · 2016

Aims: The present study was undertaken to determine the effects of drying and salting on the nutrient content and sensory properties of Talinum triangulare leaves. Study Design: The methods of drying and salting were: oven drying to constant weight at 40°C without any pretreatmen...

Open access Research Article 10.9734/BBJ/2016/21788

Waste Oil Biodegradation Potential among Bacteria Isolates from Waste Oil and Pristine Soils

D. R. Tiku & B. E. Asikong · Biotechnology Journal International · 2016

The research study was aimed at investigating waste oil biodegradation potential among bacteria isolates from waste oil and pristine soil samples. Auto-mechanic workshop and pristine soil environments were randomly sampled within Calabar Metropolis. The study was completed within...

Open access Research Article 10.9734/BBJ/2016/23598

Mixed Palm Oil Waste Utilization through Integrated Mushroom and Biogas Production

Stella Gilbert Temu, Anselm P. Moshi, Ivo Achu Nges, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi & Bo Mattiasson · Biotechnology Journal International · 2016

Aim of the Study: The study was to integrate mushroom and biogas production using mixed palm oil to provide both food and energy source to palm oil producing communities as well as reducing environmental pollution. Design of the Study: Mixed palm oil waste was divided into two po...

Open access Research Article 10.9734/BBJ/2016/23385

Essential Oil Constituents from the Fruit of Alpinia menghaiensis

Do N. Dai, Le T. Huong, Tran D. Thang, Tajudeen O. Olayiwola, Abdul Razaq A. Ogunmoye & Isiaka A. Ogunwande · Biotechnology Journal International · 2016

The chemical composition of volatiles from the fruits of Alpinia menghaiensis S. Q. Tong & Y. M. Xia (Zingiberaceae) has been studied. The essential oils were obtained by hydrodistillation in a Clevenger-type apparatus and analyzed by gas chromatography (GC-FID) and gas chrom...

Open access Research Article 10.9734/BBJ/2016/22908

Jumping Genes-“The Other Half of the Human Genome” and the Missing Heritability Conundrum of Human Genetic Disorders

Deepu Pandita & Anu Pandita · Biotechnology Journal International · 2016

Almost 45% of the human genome is composed of transposons with about 4 million copies. The retrotransposons constitute 42% of the human genome with active families (Long Interspersed Nuclear Element, SINE-R, VNTR, and Alu) allied with insertional mutagenesis and diseases. There a...

Open access Research Article 10.9734/BBJ/2016/13904

DNA from Oral Rinse- A Comparison of Three Protocols for Amplification of VDR, FTO and Beta-Globin Genes

Muhammad Haris Lucky & Saeeda Baig · Biotechnology Journal International · 2016

Aims: The aim of the study was to evaluate the efficacy of three different protocols for DNA extraction from oral rinse on the basis of their quantity and quality of DNA, simplicity, cost effectiveness and rapidity, as well as its efficiency for amplification of vitamin D recepto...

Open access Research Article 10.9734/BBJ/2016/22199