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All published articles 904

Screening and Media Optimization for Enhancing L-asparaginase Production, an Anticancer Agent, from Different Filamentous Fungi in Solid State Fermentation

Ali Mohamed Elshafei & Dina Helmy El-Ghonemy · Biotechnology Journal International · 2015

Aim: The aim of present study was to screen new potent fungal isolates and microorganisms possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural and environmental conditions required for enzyme production will be carried out for the hig...

Open access Research Article 10.9734/BBJ/2015/19728

Cellulase Producing Potential of Aspergillus terreus Uv2 on Cellulosic Wastes Pretreated with Acid and Alkali

D. Damisa, F. A. Kuta, O. P. Abioye, J. D. Bala & N. E. Egbe · Biotechnology Journal International · 2015

Aims: Cellulases offer very wide applications in biotechnology and enzymes from microbial origins present inexpensive source. Production of value added chemicals from wastes will be an exciting translation from waste to wealth and an eco-friendly initiative instead of the inciner...

Open access Research Article 10.9734/BBJ/2015/18773

New Assay Method for Allene Oxide Cyclase, an Important Enzyme in Jasmonic Acid Biosynthesis

Keimei Oh & Yoichiro Shimura · Biotechnology Journal International · 2015

Aims: Allene oxide cyclase (AOC) (EC 5.3.99.6) is an important enzyme of jasmonates (JAs) biosynthesis. JAs are important signals that play a pivotal role in defense response of plants to environmental cues. Regulation JA biosynthesis is believed useful for elucidating the mechan...

Open access Research Article 10.9734/BBJ/2015/20089

Relationship of Genomic G+C Content between Phages/Plasmids and Their Hosts

Hiroshi Nakashima, Keiko Homma & Kazuhiro Mawatari · Biotechnology Journal International · 2015

Aims: We compared genomic G+C content between bacteriophages/plasmids and their host genomes of 46 species to reveal their relation. To examine the large deviation in the G+C content between bacteriophages and their host genomes, the ancestral bacteriophage which infected early w...

Open access Research Article 10.9734/BBJ/2015/20046

Evaluation of Genetic Variability in Adansonia digitata L. by RAPD Analysis

Laxmi Gautam, Showkat Ahmad Ganie, Rajneesh Kumar Agnihotri & Rajendra Sharma · Biotechnology Journal International · 2015

Baobab (Adansonia digitata L.) belonging to Bombacaceae family, is one of the most widely used indigenous tree species in sub-Saharan Africa. The objective of the present study was to assess molecular variation among A. digitata and to determine the level of genetic similarity am...

Open access Research Article 10.9734/BBJ/2015/18727

Isolation and Characterization of Cellulase Producing Bacteria from the Gut of Termites (Odontotermes and Heterotermes Species)

C. P. Sreena, N. K. Resna & Denoj Sebastian · Biotechnology Journal International · 2015

Aim: To identify, characterize and compare the cellulolytic potentials of strains isolated from the gut of Odontotermes and Heterotermes species. Methodology: Termites were collected, identified, surface sterilized and used as source of cellulase producers. Enrichment of cellulos...

Open access Research Article 10.9734/BBJ/2015/20001

Effect of Cooking Time on Starch and Cyanide Contents of Freshly Harvested Cassava Tubers Used for Tapioca Production

O. R. Ezeigbo, M. U. Ekaiko & Z. O. Ibegbulem · Biotechnology Journal International · 2015

This study investigated the effect of cooking time on starch and cyanide contents of freshly harvested cassava tubers used for tapioca production. Tapioca is a cassava meal commonly consumed in most part of the world and prepared from freshly harvested cassava tubers. The cassava...

Open access Research Article 10.9734/BBJ/2015/16944

Processing and Quality of “red kapsiki” an Opaque Beer from “Mandara” Mountain in Cameroon

Bayoi James, Djoulde Darman Roger, Aboubakar Dandjouma, Essia- Ngang Jean Justin & François- Xavier Etoa · Biotechnology Journal International · 2015

Aims: Mandara is a chain of Mountains located in northern Cameroun with the border of the republic of Nigeria. This area is populated by “kapsiki” an indigenous populations living. This non moslem population is brewing an opaque beer, which has both a symbolic and nutritional. Th...

Open access Research Article 10.9734/BBJ/2015/18919

Assessment of Genetic Diversity in Ethiopian Sesame (Sesamum indicum L.) Germplasm Using ISSR Markers

Mohammed Abate, Firew Mekbib, Amsalu Ayana & Mandefro Nigussie · Biotechnology Journal International · 2015

Aim: This study aimed to uncover the diversity and population structure of 128 sesame genotypes using ISSR markers and identify highly diverse genotypes for the purposes of broadening the genetic base of sesame landraces grown in Ethiopia. Place and Duration of Study: The study w...

Open access Research Article 10.9734/BBJ/2015/18481

New Monoclonal Antibodies to Human IgA: Obtaining and Study of Biological Properties

Alexander Galkin, Alex Dugan, Valentine Solovjova & Larisa Bondarenko · Biotechnology Journal International · 2015

Aim: Preparation of monoclonal antibodies to human IgA, investigation of their properties and selection of the most appropriate McAbs for highly sensitive and specific immunoassay tests. Methodology: Balb/c mice were used for monoclonal antibodies (McAbs) production. Animals were...

Open access Research Article 10.9734/BBJ/2015/18955

Production of Amylase by Arthrobacter kerguelensis VL-RK_09 Isolated from Mango Orchards

Rajesh Kumar Munaganti, Kumar Munaganti & Krishna Naragani · Biotechnology Journal International · 2015

Objectives: To optimize the cultural parameters for improved production of amylase by Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards of Vissannapet, Krishna District, A.P., India. Methods: The strain A. kerguelensis was screened initially for amylase production o...

Open access Research Article 10.9734/BBJ/2015/19383

Histo-architechtural Evaluation of Conventional Versus Two Rapid Microwave Processing Techniques

Tobias Peter Pwajok Choji, Anthony Ajuluchukwu Ngokere, Samuel Ifedioranma Ogenyi & Peterside Rinle Kumbish · Biotechnology Journal International · 2015

Aims: This work aims to reduce the time of tissue processing, exclude xylene from tissue processing as well as to reduce the total quantity of reagents used per cycle of paraffin wax processing technique. Study Design: Harvesting and fixation of tissues. Grossing into triplicates...

Open access Research Article 10.9734/BBJ/2015/18948

Development of Maize Haploid Inducer Lines and Doubled Haploid Lines in Pakistan

Khunsa Khakwani, Muhammad Rafiq Dogar, Muhammad Ahsan, Amir Hussain, Muhammad Asif, Ahsan Raza Malhi & Muhammad Altaf · Biotechnology Journal International · 2015

The work on maize doubled haploid development has started at the Maize Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan during 2010 in collaboration with University of Agriculture Faisalabad, Pakistan. The aim of current study was to develop locally ad...

Open access Research Article 10.9734/BBJ/2015/18394