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All published articles 904

Towards Efficient In vitro Regeneration of Cowpea (Vigna unguiculata L. Walp): A Review

Lawan Abdu Sani, Inuwa Shehu Usman, Muhammad Ishiaku Faguji & Sunusi Muhammad Bugaje · Biotechnology Journal International · 2015

Cowpea is a crop of tremendous economic and ecological values particularly in sub-Saharan Africa, where over 80% of the crop is produced and consumed. Due to heavy attack by pests and diseases, actual yield does not exceed 20% of the crop’s potential in most of the production reg...

Open access Research Article 10.9734/BBJ/2015/16841

Establishment of a Simple Plant Regeneration System Using Callus from Apomictic and Sexual Seeds of Guinea Grass (Panicum maximum)

Chen Lanzhuang, Nishimura Yoshiko, Umeki Kazuma, Zhang Jun & Xu Chengti · Biotechnology Journal International · 2015

Aims: For analysis of apomixis genes, as the first step, an efficient and simple plant regeneration system has been established using callus from apomictic and sexual seeds of guinea grass (Panicum maximum). Study Design: The best basic medium for callus formation from matured se...

Open access Research Article 10.9734/BBJ/2015/17215

Protective Effects of CYP2E1 Inhibitors on Metabolic Syndrome-induced Liver Injury in Guinea Pigs

Volodymyr V. Rushchak, Ganna M. Shayakhmetova, Anatoliy V. Matvienko & Mykola O. Chashchyn · Biotechnology Journal International · 2015

The present work reports the effects of CYP2E1-inhibitors (quercetin, 4- methylpyrazole and disulfiram) on the indices characterizing state of the liver in guinea pigs with metabolic syndrome (MS) induced by protamine sulfate repeated administrations. The investigation of quercet...

Open access Research Article 10.9734/BBJ/2015/17756

Plasmid and Restriction Endonuclease Analyses of Bacteria Associated with Pneumonia in HIV/AIDS Patients in Ekiti State, Nigeria

A. O. Oluyege & O. Ojo-Bola · Biotechnology Journal International · 2015

Bacterial infections are an important cause of morbidity and mortality in individuals with the human immunodeficiency virus. The emergence of multiple-drug resistant bacteria has been documented by many researches. This study was therefore carried out to determine whether the res...

Open access Research Article 10.9734/BBJ/2015/16656

Optimization of Operating Conditions Affecting Microbiologically Influenced Corrosion of Mild Steel Exposed to Crude Oil Environments Using Response Surface Methodology

K. K. Salam, S. E. Agarry, A. O. Arinkoola & I. O. Shoremekun · Biotechnology Journal International · 2015

In this study, the influence of four operating parameters (pH, salinity, nitrate concentration and immersion time) and their interactions on the microbiologically influenced corrosion (MIC) rate of mild steel in simulated crude oil environments were investigated by response surfa...

Open access Research Article 10.9734/BBJ/2015/16810

Comparing the Effect of Two Promoters on Cassava Somatic Embryo at Transient GUS Assay Level

Olufemi O. Oyelakin, Jelili T. Opabode & Emmanuel O. Idehen · Biotechnology Journal International · 2015

35S promoter from the Cauliflower Mosaic Virus (pCaMV) is a constitutive promoter commonly used in plant genetic transformation while Cassava Mosaic Virus (pCsVMV) is another promoter which is underutilized. The combination of the two promoters was used to form (pOYE153). The met...

Open access Research Article 10.9734/BBJ/2015/17092

Antibacterial Activity of Phytoalexins from Infected Theobroma cacao L

Emmanuel Dayo Fagbohun, Babatunde Idowu Aderiye & Saburi Adejimi Adesanya · Biotechnology Journal International · 2015

Aims: To study the antimicrobial activities of the compounds produced due to reactions of cocoa (Theobroma cacao) with Phytophthora palmivora during infection. Place and Duration of Study: Department of Microbiology, Ekiti State University, Ado Ekiti, Nigeria and the Department o...

Open access Research Article 10.9734/BBJ/2015/17373

Molecular Analysis of Genetic Diversity of Pectobacterium carotovorum Subsp Carotovorum Isolated in Morocco by PCR Amplification of the 16S-23S Intergenic Spacer Region

M. Amdan, H. Faquihi, M. Terta, M. M. Ennaji & R. Ait Mhand · Biotechnology Journal International · 2015

Aims: Pectobacterium carotovorum is a ubiquitous bacterium that causes soft rot in different crop plants throughout the world. In Morocco, approximately 95% of the Strains isolates from potato plants with tuber soft rot are P. carotovorum. In this study, we test whether PCR ribot...

Open access Research Article 10.9734/BBJ/2015/16045

Tolerance Potentials of Cocoa (Theobroma cacao) in Hydrocarbon Polluted Soils

R. B. Agbor, J. E. Asor, I. U. Bassey, Chibuzor Obianwa, E. O. Ude & A. Ipoh · Biotechnology Journal International · 2015

In the present study the tolerance potentials of T. cacao in hydrocarbon polluted soil was evaluated. Top soil (0-25 cm depth) was collected from three points, bulked to form composite soil sample. Eight kilograms of the soil sample each were weighed into twenty five (25) perfora...

Open access Research Article 10.9734/BBJ/2015/13863

Regeneration Procedure for Three Arachis hypogaea L. Botanicals in Uganda through Embryogenesis

D. K. Okello, L. B. Akello, P. Tukamuhabwa, S. M. Ochwo, T. L. Odong, J. Adriko, C. Mwami & C. M. Deom · Biotechnology Journal International · 2015

Aims: A procedure was developed for embryogenesis from embryo explants derived from mature seeds of freshly harvested Serenut 4T, Serenut 1R and Acholi-white groundnut cultivars representing the three broad groundnut botanical classifications. Methodology: This study explored the...

Open access Research Article 10.9734/BBJ/2015/17418

Screening and Production of Biodegradable Polyester Poly(3-hydroxybutyrate) by Bacteria Endophytic to Brassica nigra L.

Rituparna Das, Arundhati Pal, Sukhendu Mandal & A. K. Paul · Biotechnology Journal International · 2015

Aims: Poly(3-hydroxybutyrate) [P(3HB)], the microbially produced biodegradable thermoplastics has find wide range of applications in recent years. Development of low cost production strategies utilizing novel organisms is a crucial challenge. Present study is aimed to isolate and...

Open access Research Article 10.9734/BBJ/2015/16750

Isolation and Identification of Cellulytic Fungi from Agrowastes and Sawmill Soils

Ekundayo Temitope Cyrus & Arotupin Daniel Juwon · Biotechnology Journal International · 2015

Five agricultural wastes and sawmill soil were investigated for fungi capable of producing cellulase. Isolation was carried using serial dilution techniques and isolates were screened for cellulase production on carboxymethyl cellulose-containing agar plates. Copious isolates wer...

Open access Research Article 10.9734/BBJ/2015/17575

Alkaline Cellulase Production by Penicillium mallochii LMB-HP37 Isolated from Soils of a Peruvian Rainforest

Karin Vega, Victor Sarmiento, Yvette Ludeña, Nadia Vera, Carmen Tamariz-Angeles, Gretty K. Villena & Marcel Gutiérrez-Correa · Biotechnology Journal International · 2015

Alkaline cellulases are demanded by the textile industry for several purposes but commercial preparations showing activity at alkaline conditions are very scarce. Aim: To characterize a Penicillium strain isolated form soils of a Peruvian rainforest showing alkaline cellulase act...

Open access Research Article 10.9734/BBJ/2015/17831

Highlighting the Degradation of Actin in Longissimus dorsi Muscle of Different Species: Bovine, Ovine, Caprine, Poultry and Freshwater Fish

Hasina Rasolofoharitseheno, Wisdom Michael Mompi, Yasmine Boudida & Mouhammed Gagaoua · Biotechnology Journal International · 2015

This research is aimed at highlighting the degradation of actin in the Longissimus dorsi muscle of five species, namely, bovine, ovine, caprine, poultry, and freshwater fish. To reveal the degradation of actin, by Western blotting, the myofibrillar proteins of the five studied sp...

Open access Research Article 10.9734/BBJ/2015/17054

Haematological Response and Serum Biochemical Profile of Broiler Finishers Fed with Oxytetracycline and Stonebreaker (Phyllanthus amarus) Leaf Meal

A. D. Ologhobo & I. O. Adejumo · Biotechnology Journal International · 2015

Aims: The aim of the study was to assess the effect of Phyllanthus amarus leaf meal on haematology and serum biochemical profile of broiler finishers vis a vis oxytetracycline, in order to serve a basis for further study focusing on antibacterial properties of Phyllanthus amarus....

Open access Research Article 10.9734/BBJ/2015/10304