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All published articles 1739

Phytochemical Analysis and Chromatographic Studies of Pergularia tomentosa L. and Mitracarpus scaber Zucc

S. A. Shinkafi · Microbiology Research Journal International · 2014

Qualitative phytochemical analysis was carried out on Pergularia tomentosa and Mitracarpus scaber (leaves), the results revealed the presence of Tannins, Flavonoids, Alkaloids, Saponins, Glycosides, Saponin Glycosides, Cardiacglycosides, Anthraquinone and Steroids.The quantitativ...

Open access Research Article 10.9734/BMRJ/2014/3534

Direct and Rapid Diagnosis of Extra-Pulmonary Tuberculosis in Archival Tissue by Nested and Touchdown PCR

L. F. Nimri, Z. Al-joulani & I. Matalka · Microbiology Research Journal International · 2014

Aims: To identify and differentiate mycobacterium spp. in archival formalin-fixed, paraffin-embedded tissue by PCR to supply additional differential diagnostic method for Mycobacterium infections, where tuberculosis had been tested for by histopathological methods but without cul...

Open access Research Article 10.9734/BMRJ/2014/8427

A Review of Fusobacterium necrophorum Infections in Humans

Emelda E. Chukwu, Francisca O. Nwaokorie & Akitoye O. Coker · Microbiology Research Journal International · 2014

The incidence of human infections caused by Fusobacterium necrophorum is recently on the increase and this is attributed largely to alteration in antibiotic usage pattern, malnutrition and poor oral hygiene. These infections are usually acquired exogenously from animals such as d...

Open access Research Article 10.9734/BMRJ/2014/6634

The Evaluation of the Combination of Caspofungin with Voriconazole and Amphotericin B against Clinical Candida krusei Isolates by Etest and Disk Diffusion Methods

Yasemin Oz, Abdurrahman Kiremitci, Filiz Aksit & Nuri Kiraz · Microbiology Research Journal International · 2014

Background: C. krusei is an opportunistic fungal pathogen that known of its intrinsic fluconazole resistance and its frequency is increasing especially among hematology patients. The increase in the frequency of high mortality fungal infections have accelerated the efforts of new...

Open access Research Article 10.9734/BMRJ/2014/6950

Enzymatic Activity of Lactobacillus Grown in a Sweet Potato Base Medium

Saeed A. Hayek, Aboghasem Shahbazi, Mulumebet Worku & Salam A. Ibrahim · Microbiology Research Journal International · 2014

Aim: To study the impact of sweet potatoes on α-glucosidase, β-glucosidase, acid phosphatase, and phytase activity of Lactobacillus. Study Design: Enzymatic activity of seven strains of Lactobacillus grown in a sweet potato medium (SPM) was determined and compared to the standard...

Open access Research Article 10.9734/BMRJ/2014/5921

In-vitro Effect of Iron on the Growth Density of Mycobacterium tuberculosis and Drug Susceptibility Testing

Rasheeda H. Abdalla, Ali M. S. Eleragi, Salaheddin O. Hassan, Nuha Y. Ibrahim & Mohamed S. El Sanousi · Microbiology Research Journal International · 2014

This work was carried out to study the effect of iron on the growth density of Mycobacterium tuberculosis as well as drug susceptibility testing. Fifty two smear-positive acid fast bacilli out of 100 sputum specimens were obtained from patients who were referred to National Healt...

Open access Research Article 10.9734/BMRJ/2014/7049

Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-tafilalet Soils and Study of Their Ability to Nodulate Bituminaria bituminosa

Btissam Ben Messaoud, Imane Aboumerieme, Laila Nassiri, Elmostafa El Fahime & Jamal Ibijbijen · Microbiology Research Journal International · 2014

Aims: The objectives were to isolate and characterize phenotypically and genotypically the rhizobial strains from the soils belonging to the Meknes-Tafilalet region in order to select strains that are able to nodulate Bituminaria bituminosa. Study Design: An experimental study. P...

Open access Research Article 10.9734/BMRJ/2014/6172

Salmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens

Anwar Ali Abdulla, Mokhtar Arshad & A. Mahdi Saeed · Microbiology Research Journal International · 2014

Aims: To study the effect of flagellin on bacterial attachment and invasion of avian ovary cells in vitro by comparing the attachment and invasion of wild-type S. Enteritidis with non-motile mutants. To assess the immunogenic properties of extracted flagellin against Salmonella E...

Open access Research Article 10.9734/BMRJ/2014/7425

Optimization of Process Parameters for Improved Production of Bioactive Metabolites by Streptomyces tritolerans DAS 165T

Usha Kiranmayi Mangamuri, Vijayalakshmi Muvva, Sudhakar Poda & Dayanand Agasar · Microbiology Research Journal International · 2014

Aims: To optimize the process parameters for enhanced production of bioactive metabolites by Streptomyces tritolerans DAS 165T. Place and Duration of Study: Department of Botany and Microbiology, April 2012 to August 2012. Methodology: Agar well diffusion assay was employed to st...

Open access Research Article 10.9734/BMRJ/2014/7086

Rapid Detection of Extended Spectrum Β-lactamase (ESBL) Producing Strain of Escherichia coli in Urinary Tract Infections Patients in Benha University Hospital, Egypt

Enas Sh. Khater & Hammouda W. Sherif · Microbiology Research Journal International · 2014

Background: An increase in extended spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) has been observed. Aims: Of this study was done to detect the prevelance of ESBL, AmpC producing and ESBL and AmpC co-producing strains of Escherichia coli (E. coli) in urinary tr...

Open access Research Article 10.9734/BMRJ/2014/7376

Determination of Vancomycin and Methicillin Resistance in Clinical Isolates of Staphylococcus aureus in Iranian Hospitals

Abbas Farhadian, Qorban Behzadian Nejad, Shahin Najar peerayeh, Mohammad Rahbar & Farzam Vaziri · Microbiology Research Journal International · 2014

Aims: The aim of this study was to determine the prevalence of methicillin resistant S. aureus (MRSA), vancomycin resistant or vancomycin intermediate resistant S. aurues (aureus) (VRSA/VISA) among clinical isolates. Study Design: S.aureus isolates used in this study were randoml...

Open access Research Article 10.9734/BMRJ/2014/4836

Prevalence and Antibiotic Resistance Profile of Salmonella Isolates from Commercial Poultry and Poultry Farm-handlers in Jos, Plateau State, Nigeria

G. O. A. Agada, I. O. Abdullahi, M. Aminu, M. Odugbo, S. C. Chollom, P. R. Kumbish & A. E. J. Okwori · Microbiology Research Journal International · 2014

Aim: This study was designed to investigate the prevalence and antibiotic resistance profile of Salmonella serovars from poultry and poultry farm-handlers. Study Design: Investigative Place and Duration of Study: Samples were analyzed at the Central Diagnostic Laboratory, Nationa...

Open access Research Article 10.9734/BMRJ/2014/5872

Lipolytic Activity and Molecular Identification of Pseudomonas aeruginosa and Lysinibacillus sphaericus Isolated from Domestic Oil Rich Wastewater

A. T. Odeyemi, B. I. Aderiye, E. I. Adeyeye, E. Donbraye & T. Faleye · Microbiology Research Journal International · 2014

Microbial lipases have been heightened in bioremediation and various industries. Place and Duration of Study: Department of Microbiology, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria, between September 2010 and August 2011. To identify the lipolytic enzyme producing mi...

Open access Research Article 10.9734/BMRJ/2014/6587

Listeria spp. and Enterobacteriaceae Group in Sandwiches of Meat and Meat Products

Rashed A. Zaghloul, Moustafa A. El-Shenawy, Naseem A. Neweigy, Hamed E. Abou-Aly, Raouf K. El-dairouty, Wagih I. El-Kholy, Mohamed T. Fouad, J. M. Soriano, J. Mañes & Lydia Micó · Microbiology Research Journal International · 2014

Aims: To evaluate the Egyptian street-vended sandwiches of meat and meat products for the presence of Listeria monocytogenes and Enterobacteriaceae group. To evaluate the microbiological quality of street-vended sandwiches of meat and meat products sold in Cairo-Egypt. Place and...

Open access Research Article 10.9734/BMRJ/2014/7417

Isolation, Identification and Optimization of Culture Conditions of Bacillus sp. Strain PM1 for Alkalo-thermostable Amylase Production

Kinshuk Sharma, Sarita Bhutty, S. M. Paul Khurana & Urvashi Kumar Kohli · Microbiology Research Journal International · 2014

Aims: To isolate and optimize the culture conditions for thermo stable and alkaline amylase production from bacteria. Study Design: Optimization of different physiological and nutritional parameters for amylase production and kinetic studies of amylase. Place and Duration of Stud...

Open access Research Article 10.9734/BMRJ/2014/6873