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Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

Iserhienrhien Lucky Osafanme & Okolie Paulinus Ngozi · Asian Journal of Research in Biochemistry · 2018

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydraz...

Open access Research Article 10.9734/ajrb/2018/v3i229823

Separation and Optimisation of a Sucrose Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells (PBMC)

Sudeep Nagaraj, Shubha Nivargi, Leelavathy Nanjappa & Jagadish Tavarekere Venkataravanappa · Asian Journal of Research in Biochemistry · 2018

One step centrifugation procedure used commonly for separation of blood cells is the ficoll gradient centrifugation. In this method, after centrifugation, the peripheral blood mononuclear cells (PBMCs) are located on the top of the separation fluid, whereas other blood cells eryt...

Open access Research Article 10.9734/ajrb/2018/v3i229820

Ameliorative Effect of Methanol Extract of Hymenocardia acida Leaves on Gentamicin-Induced Renal Toxicity in Rats

Nweje-Anyalowu Paul Chukwuemeka, Idakwoji Precious Adejoh, Iserhienrhien Lucky Osafanme & Anosike Joy Chizoba · Asian Journal of Research in Biochemistry · 2018

Aim: This study evaluated the nephroprotective effect of methanol extract of Hymenocardia acida leaves in rat model of gentamicin induced renal damage. Materials and Methods: Twenty- four (24) Wistar albino rats of either sex weighing 150- 200g were divided into 4 groups of 6 ani...

Open access Research Article 10.9734/ajrb/2018/v3i229818

Biochemical Alterations in the Liver and Kidney of Rats Following Sub-acute Administration of Aqueous Extract of Stem-bark of Anacardium occidentale (Cashew Tree)

Ademola C. Famurewa, Funmilayo Showunmi, Abiola M. Folawiyo, Michael Epete, Paul I. Okike & Maxwell C. Onuoha · Asian Journal of Research in Biochemistry · 2018

Background: The use of herbal medicines for treating ailments is rampant in recent years, and the toxicity implications of various plant preparations are sparingly reported. We investigated the potential effect of daily administration of aqueous extract of stem-bark of cashew tre...

Open access Research Article 10.9734/ajrb/2018/v3i129815

Toxoplasma gondii and Tuberculosis Co-infection among Tuberculosis patients at the Bamenda Regional Hospital, North West Region, Cameroon

Wanabo M. Kita & Christopher B. Tume · Asian Journal of Research in Biochemistry · 2018

Aim: To determine the prevalence of toxoplasmosis and tuberculosis co-infection among (TB) patients.  Study Design: A cross-sectional study was employed to gather data on 147 sputum positive TB patients. Place and Duration of Study:  Bamenda Regional Hospital (BRH), Tuberculosis...

Open access Research Article 10.9734/ajrb/2018/v3i129812

Intestinal Microbiota and Immunity: A Review

C. F. Egwuonwu, E. C. Evans & A. L. Olokun · Asian Journal of Research in Biochemistry · 2018

Microorganisms establish a symbiotic relationship at all stages of growth in man, beginning from birth to adulthood. They are found in every part of the body, and they participate in the immune system against pathogen-mediated immune responses. Their actions are elicited by secre...

Open access Research Article 10.9734/ajrb/2018/v3i129810

Electrolyte Concentrations in Apparently Healthy Individuals after Consumption of Aqueous Extract of Jatropha tanjorensis

Ibiene Sarah Kalio & Okorie Akuegbo Ezinne · Asian Journal of Research in Biochemistry · 2018

Jatropha tanjorensis is a herbaceous plant used locally for the treatment of diabetes, inflammation, and stomach ache in Nigeria. Longtime effect of Jatropha tanjorensis on electrolyte concentrations in the body is yet to be determined. This study was conducted to determine elect...

Open access Research Article 10.9734/ajrb/2018/v3i129807

Antifungal Potentials of Acacia nilotica, Ziziphus jujube Linn and Lawsonia inermis

A. L. Abubakar, A. Dandare, U. F. Magaji, I. H. Abubakar, M. Yerima & R. S. U. Wasagu · Asian Journal of Research in Biochemistry · 2018

Background: The increasing emergence of resistance to conventional antimicrobial drugs and the complicity of their usage is a serious challenge in Nigeria. In our previous report, it was demonstrated that methanol leaves extracts of Acacia nilotica, Ziziphus jujube Linn and Lawso...

Open access Research Article 10.9734/ajrb/2018/v3i11057

High-level Expression and Purification of DNA and DNase Free Taq DNA Polymerase

Mukti Nath Mishra, Jeevanandam Mohanraj, S. Durairaju Nisshanthini & Shama Bhat · Asian Journal of Research in Biochemistry · 2018

Aim: Taq DNA polymerase from Thermus aquaticus is a key enzyme in the field of molecular biology that has been mostly used in polymerase chain reaction (PCR). Our aim is to produce standard grade Taq DNA polymerase free from censorious impurities like DNase, DNA and other contami...

Open access Research Article 10.9734/ajrb/2018/v2i4607

Effect of Ethanol Extract of Cucurbita pepo Leaves on the Lipid Profile of Wistar Albino Rats

F. U. Eneh, G. C. Ugochukwu & C. M. Okoye · Asian Journal of Research in Biochemistry · 2018

Aim: The role of nutrition in lipid metabolism continues to generate a lot of research interest, especially as dyslipidaemia is implicated in a host of diseases. The work investigated the effect of ethanol extract of  Curcubita pepo leaves on the lipid profile of Wister rats. Met...

Open access Research Article 10.9734/ajrb/2018/v2i4601

Evaluation of Heavy Metals in Fish, Water and Sediment of the New Calabar River

Amechi, Prince Aruchi Chikordi, Wegwu, Matthew Owhonda, Omeodu, Steve Ike & Felagha, Iniebiyo · Asian Journal of Research in Biochemistry · 2018

Aim: This study is aimed at determining the levels of some heavy metals in the water, sediment and fishes (Oreochromis niloticus, Periopthalmus koelreuteri, Micropogonias undulatus, Carassius auratus and Liza felcipinis) from new Calabar River located at Rumuolumeni Community in...

Open access Research Article 10.9734/ajrb/2018/v2i4599

Toxicity Activity-Guided Characterization of Toxic Constituents in Azadirachta indica Seed

Edwin N. Okafor, O. U. Njoku & Okoli Charles · Asian Journal of Research in Biochemistry · 2018

Background: Azadiradita indica seed has been used in traditional system of medicine. It is known to be natural medicine with many benefits. Toxicological studies have reported that toxic effects may be related to the complex mixtures of active constituents and other chemicals whi...

Open access Research Article 10.9734/ajrb/2018/v2i4561

Cholesterol-Lowering Effects of Lactobacilli and Bifidobacteria Probiotics in vitro and in vivo

Shahenda, M. Elaby, Asmaa A. Salem, Jehan, B. Ali & A. F. Abdel-Salam · Asian Journal of Research in Biochemistry · 2018

Two lactobacilli strains; Lactobacillus acidophilus ATCC 20079 and Lactobacillus plantarum ATCC 20179 and two bifidobacteria strains; Bifidobacterium bifidum GSGG 5286 and Bifidobacterium longum ATCC 15707 were studied their abilities to reduce the cholesterol content in vitro. I...

Open access Research Article 10.9734/ajrb/2018/v2i4540

In vitro Potential of Aqueous Extracts of Plant Leaves to inhibit Pathogenic Fungi

Liamngee Kator & Zakki Yula Hosea · Asian Journal of Research in Biochemistry · 2018

The in vitro potential of aqueous extracts of plant leaves to inhibit pathogenic fungi was carried out. The effect of leaf extract and concentration on growth inhibition of organism I (Aspergillus flavus) in vitro revealed that a concentration of 100g/mL, Moringa oleifera leaf ex...

Open access Research Article 10.9734/ajrb/2018/v2i3535

Ethnomedicinal Use, Phytochemical Constituents and Bioactivity of Wild Edible Fruits Commonly Consumed in Burkina Faso

Ablassé Rouamba, Moussa Compaoré & Martin Kiendrebeogo · Asian Journal of Research in Biochemistry · 2018

The screening of bioactive phytochemical constituents in edible fruits and vegetable is interesting to researchers owing their benefit property on consumer’s health. Fruits are used ethnomedicinally to treat numerous diseases including metabolic and microbial diseases. They are a...

Open access Research Article 10.9734/ajrb/2018/v2i3530