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All published articles 201

Complementary Amino Acid Profile of Dacryodes edulis Fruit and Zea mays Grain

EZENNAYA, C. F., IGWENYI, I. O., EZEIGWE O. C., OGUNWA, S. C. & NWOBODO, A. M. · Asian Journal of Biotechnology and Genetic Engineering · 2025

Amino acids are vital biomolecules that serve as building blocks of proteins and perform diverse physiological and metabolic functions in living organisms. Plants are a major source of protein especially in regions of food insecurity. This study evaluated and compared the amino a...

Open access Research Article 10.9734/ajbge/2025/v8i2173

Advances in CRISPR-Cas9 Genome Editing: Mechanisms, Variants, Delivery Systems, and Biomedical Applications

Raja Sekhar Yeluri, Navya Sri Gorli & Sita Kumari Karanam · Asian Journal of Biotechnology and Genetic Engineering · 2025

The CRISPR-Cas9 technology and related programmable nucleases have transformed genome editing by enabling precise and targeted DNA modifications in eukaryotic cells. Compared with earlier tools such as zinc-finger nucleases and TALENs, Cas9 provides higher efficiency and programm...

Open access Research Article 10.9734/ajbge/2025/v8i2172

Isolation and Characterization of Microorganisms Associated with Bread Spoilage around Clifford University, Ihie, Abia State, Nigeria

Ifeanyi B. Ezea, Ibe-Sharon D. Chiugo & Ngozi B. Ezeugwu · Asian Journal of Biotechnology and Genetic Engineering · 2025

Bread products are among the most important basic foods consumed by people around the world. They are widely recognized as a highly convenient type of food that appeals to all populations, rich and poor, rural and urban. Bread products are vulnerable to a variety of spoilage arou...

Open access Research Article 10.9734/ajbge/2025/v8i2171

Identification of Naturally Occurring Biflavonoids as Potential BCL-2 Inhibitor: A Computational Approach

Esther O. Shalom, Esther A. Olanudun, Josephine O. Fagbemi, Catherine O. Ajibola, Iyanu O. Awotuya, Boluwaji I. Makinde, Imisioluwa A. Akintola & Kolade O. Faloye · Asian Journal of Biotechnology and Genetic Engineering · 2025

B-cell lymphoma 2 (BCL-2) is a protein that enhances malignant cell survival in the pathophysiology of cancer. Biflavonoids are naturally occurring phytochemicals with diverse therapeutic benefits. This study investigated the BCL-2 inhibitory potential of C-O-C linked biflavonoid...

Open access Research Article 10.9734/ajbge/2025/v8i2170

Micropropagation Via Embryogenesis Techniques for Reforestation of Valuable Teak (Tectona grandis Linn. f.)

Tran Van Minh, Pham Hong Diep & Bui Thanh Hoa · Asian Journal of Biotechnology and Genetic Engineering · 2025

Introduction: Teak is a valuable woody timber trees in industry. Aim: Teak (Tectona grandis Linn. f.) is an important valuable timber value. Teak trees grow slowly with a harvesting cycle of 15-20 years and the seed germination rate is low. tissue culture is a method of rapid pro...

Open access Research Article 10.9734/ajbge/2025/v8i2169

Comparative Study of Hematological and Renal Profiles in Rats Fed Hibiscus sabdariffa (Zobo) Drink Sweetened with Natural Versus Artificial Sugars

Owhonda Golden Chukwuemeka, Wokocha Peter Gift, Okari Karibo Amakiri, Opusunju Boma Harris & Oluoha Uchechukwu Raphael · Asian Journal of Biotechnology and Genetic Engineering · 2025

Hibiscus sabdariffa (commonly known as "zobo") is widely consumed for its antioxidant, antihypertensive, and nephroprotective effects. However, the impact of sweeteners—natural (honey) vs. artificial (aspartame)—on these benefits remains underexplored. This study assessed hematol...

Open access Research Article 10.9734/ajbge/2025/v8i2168

Comparative Analysis of the Hepatoprotective and Hematopoietic Effects of Moringa oleifera and Gongronema latifolium Extracts in Protein-Deficient Rats

Oluoha Uchechukwu Raphael, Wokocha Peter Gift, Okari Karibo Amakiri, Opusunju Boma Harris & Owhonda Golden Chukwuemeka · Asian Journal of Biotechnology and Genetic Engineering · 2025

Protein malnutrition is a major contributor to hepatic and hematopoietic dysfunction, particularly in vulnerable populations. This study aimed to comparatively evaluate the hepatoprotective and hematopoietic effects of Moringa oleifera and Gongronema latifolium leaf extracts in W...

Open access Research Article 10.9734/ajbge/2025/v8i2167

Optimizing Geneticin Lethal Dose for Effective NPT-II Selection in Transgenic Sugarcane (Genotype CoC671)

Satish Deole & Anil Kulkarni · Asian Journal of Biotechnology and Genetic Engineering · 2025

Establishing a kill curve is an essential preliminary step in the genetic transformation of plants, including sugarcane. This process helps determine the optimal selection pressure required to differentiate between transformed and non-transformed tissues, ensuring that only genet...

Open access Research Article 10.9734/ajbge/2025/v8i2166

Micropropagation Under Pilot-Scale for Reforetation of Endangered Special Agarwood Species (Aquilaria crassna Pierre ex Lecomte)

Dinh Trung Chanh, Pham Hong Diep, Bui Thanh Hoa & Tran Van Minh · Asian Journal of Biotechnology and Genetic Engineering · 2025

Introduction: Agarwood is a valuable and rare and valuable wood species. Planting Aquilaria to harvest Agarwood and Ky Nam. Methods: It is successfully in micropropagation. Results: WPM is a basic media, supplemented with BA (0.1 mg/l), NAA (0.1 mg/l), coconut water (10 %), high...

Open access Research Article 10.9734/ajbge/2025/v8i2165

Quantitative and Qualitative Phytochemical Analysis of Ethanoic Leaf Extract of Mentha piperita

D. K Ogbuokiri & A.L Asomugha · Asian Journal of Biotechnology and Genetic Engineering · 2025

Mentha piperita (peppermint) is a widely used medicinal plant known for its therapeutic properties, yet its neurobiological and toxicological safety profile remains under-explored in animal models. This study investigates the phytochemical constituents, elemental composition, and...

Open access Research Article 10.9734/ajbge/2025/v8i2163

Genotyping and Antifungal Susceptibility Profile of Candida albicans Isolated from HIV-infected Women in Niamsey, Niger

Abdoul Aziz Ardaly Djibo, Gyar Silas Dogara, Abdourahmane Yacouba, Alhousseini, Daouda, Inouss Issa Ali, Ounoussa Tapha, Pascal D Sedor, Ruth Fassema & Mounkaila Boutchi · Asian Journal of Biotechnology and Genetic Engineering · 2025

Aims: C. albicans infection is one of the most frequent mycoses among HIV-positive women, posing a serious health risk and significant economic burden for its management. The aim of this study was to genotype and assess the fluconazole susceptibility of C. albicans in women infec...

Open access Research Article 10.9734/ajbge/2025/v8i2164

Effect of Methionine-Supplemented Commercial Feed on the Growth Performance of Sangkuriang Catfish (Clarias gariepinus)

Elva Yenizar, Ibnu D. Buwono, Roffi Grandiosa. H & Iskandar · Asian Journal of Biotechnology and Genetic Engineering · 2025

Methionine is a crucial essential amino acid for fish, serving as the first limiting amino acid in many commercial aquafeeds. It plays a vital role in protein biosynthesis, acts as a methyl group donor in key metabolic pathways, and supports the synthesis of enzymes, hormones, an...

Open access Research Article 10.9734/ajbge/2025/v8i2162

Enhancing Colour Brightness in Barbir Fish (Puntius conchonius) Trough Dietary Supplementation with Pumpkin (Cucurbita moschata) Flour

Dinda Tazkia Putri, Ibnu Dwi Buwono, Kiki Haetami & Roffi Grandiosa · Asian Journal of Biotechnology and Genetic Engineering · 2025

This study aimed to evaluate the effect of adding pumpkin (Cucurbita moschata) flour to commercial feed on the colour brightness of barbir fish (Puntius conchonius), as well as to determine the optimal supplementation dose for maximum colour enhancement. The experiment was conduc...

Open access Research Article 10.9734/ajbge/2025/v8i1161

Absence of Validated Artemisinin Combination Therapy Resistance Markers in High and Low Malaria Transmission Seasons in Kano and Jigawa States, Nigeria

Mahmud Safiyanu, Yayo Abdulsalami Manu, Adamu Jibrin Alhasan & Abdullahi Abdulkadir Imam · Asian Journal of Biotechnology and Genetic Engineering · 2025

Introduction: The first line treatment of uncomplicated malaria (ACT) is under threat due to evolution of drug-resistant P. falcifarum species. Five amino acid on pfk13 propeller domain has been established to account for ACT resistance alongside candidate mutations in some Asian...

Open access Research Article 10.9734/ajbge/2025/v8i1160

Molecular Detection of Herpes Simplex 1, 2 and Human Cytomegalovirus in Chronic Periodontitis Patients in Khartoum State, Sudan

Husham M Taha Aloob, Dalal Abdelrahaman Noreldin Ahmed, Abdelhakam H Ali & Khalid Abdullah Enan · Asian Journal of Biotechnology and Genetic Engineering · 2025

Background: Herpes virus infected periodontal sites tend to show tissue breakdown more frequently than herpes virus free sites, and this active infection is associated with increased risk of progressive periodontal disease, Herpes viruses can reduce the host defense system via in...

Open access Research Article 10.9734/ajbge/2025/v8i1159