DNA extraction

Molecular Characterization of Bacteria Isolates from Farm-Raised Catfish Clarias gariepinus (Burchell, 1822)

A. A. Akinyemi & O. O. Oyelakin · Microbiology Research Journal International · 2014

Selected bacterial isolates from skin, gut and gills of Clarias gariepinus were collected from five fish farms at Ijebu Ode. The isolates were assessed using 16S rRNA gene sequencing method to identify them and to construct the phylogenetic relationship. A total of 10 isolates we...

Open access Research Article 10.9734/BMRJ/2014/11996

An Improved Protocol for Extraction of Metagenomic DNA from High Humus, Alkaline and Saline Soil of Chinampa for T-RFLP Fingerprinting Analysis

Salvador Embarcadero-Jiménez, Feng Long Yang, Raquel Freye-Hernández, Yanelly Trujillo-Cabrera, Flor N. Rivera Orduña, Hong Li Yuan & En Tao Wang · Microbiology Research Journal International · 2014

Aims: The principle aim of this study was to obtain high quality metagenomic DNA from the high humus-containing, alkaline soils of the chinampas, an artificial sustainable agro-ecosystem. Study Design: Different protocols reported previously were tested and were modified to extra...

Open access Research Article 10.9734/BMRJ/2014/9670