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Metagenomics

Papers (4)

Microbiomes as Natural Allies: Towards Sustainable Farming Practices

Durva Bisht, Aditi Verma, Palak Sardana & Shilpa Kaushal · Microbiology Research Journal International · 2025

The sustainability of global agriculture is increasingly threatened by climate change, soil degradation, declining biodiversity, and the overuse of chemical fertilizers and pesticides. In this context, plant- and soil-associated microbiomes have emerged as vital allies for achiev...

Open access Research Article 10.9734/mrji/2025/v35i121670

Comparative Bacterial Metagenomics of Cnidoscolus aconitifolius (Mill.) I. M. Johnston and Other Leafy Vegetables

Nkechi Gloria Ogbuji & Eromosele Anthony Ataga · Microbiology Research Journal International · 2021

Aims: Vegetables provide a favourable habitat for diverse populations of microorganisms. Some vegetables, especially the ones used in salads are ready-to-eat food products and some phyllosphere bacteria might contribute to the prolonged presence of human food-borne pathogens in t...

Open access Research Article 10.9734/mrji/2021/v31i730333

Optimization of Soil DNA Extraction Protocol Using Na2EDTA, SDS, Heating, Vortexing and CaCl2 and Its Validation for Metagenomic Studies

Malik Ahmed Pasha, Sachin A. More & P. U. Krishnaraj · International Journal of Environment and Climate Change · 2020

Aim: Several methods described previously for isolation and purification of soil DNA. Most of these protocols use combination of techniques or methods but the role and contribution of each individual method or component used is not clearly discussed. This study aims at analysing...

Open access Research Article 10.9734/ijecc/2020/v10i630201

An Improved Protocol for Extraction of Metagenomic DNA from High Humus, Alkaline and Saline Soil of Chinampa for T-RFLP Fingerprinting Analysis

Salvador Embarcadero-Jiménez, Feng Long Yang, Raquel Freye-Hernández, Yanelly Trujillo-Cabrera, Flor N. Rivera Orduña, Hong Li Yuan & En Tao Wang · Microbiology Research Journal International · 2014

Aims: The principle aim of this study was to obtain high quality metagenomic DNA from the high humus-containing, alkaline soils of the chinampas, an artificial sustainable agro-ecosystem. Study Design: Different protocols reported previously were tested and were modified to extra...

Open access Research Article 10.9734/BMRJ/2014/9670