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multiplex PCR

Papers (7)

Rapid Differential Detection of Selected Mycobacterial Species from Faecal Samples of Cattle and Buffaloes by Using In-house Designed Multiplex PCR, IS900 PCR and TaqMan Real-time PCR

Derhasar Brahma, Deepti Narang, Mudit Chandra, Sikh Tejinder Singh & Gursher Singh Sidhu · Microbiology Research Journal International · 2025

Aim: Mycobacterial infections due to Mycobacterium avium paratuberculosis (MAP) (Paratuberculosis), and M. bovis (Tuberculosis) in dairy animals are potential public health risk that needs to be diagnosí at the earliest for effective prevention. In addition, M. smegmatis (nontube...

Open access Research Article 10.9734/mrji/2025/v35i21540

Molecular Identification of Virulence Genes of Escherichia coli Isolated from Cow Milk and Its Products in Abuja, Nigeria

E. C. Okechukwu, E. U. Amuta, G. M. Gberikon, N. Chima, B. Yakubu, J. C. Igwe & M. Njoku · Microbiology Research Journal International · 2020

Shiga toxin-producing Escherichia coli have been identified as an emerging foodborne pathogen which portends serious risk to human health. Cow milk and its products are potential sources of shiga toxin-producing Escherichia coli. A relatively small number from the family of shiga...

Open access Research Article 10.9734/mrji/2020/v30i630226

Molecular Screening of Fungal Isolates of Palm Oil from South Eastern Nigeria for Aflatoxin and Ochratoxin Biosynthetic Genes Using Multiplex Polymerase Chain Reaction (mPCR)

I. N. Nwachukwu, E. S. Amadi, U. C. Ogwo, S. I. Umeh, C. C. Opurum, E. C. Chinakwe, N. U. Nwaogwugu, I. E. Ochiagha, H. D. Ogbuagu & F. N. Ujowundu · Microbiology Research Journal International · 2019

In Nigeria and many other developing countries of the world, the incidence of mycotoxin- contamination of foods and food products has attracted attention and stirred a lot of concern for food safety. This work aims at detection of aflatoxigenic and ochratoxigenic synthetic genes...

Open access Research Article 10.9734/mrji/2019/v28i530142

Identification through Culture and Molecular Methods of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus in Surface Waters in Rasht

Keyvan Roshanjo, Nematallah Jonaidi Jafari, Leila Asadpour, Reza Ranjbar, Davoud Afshar, Abbas Farahani, Milad Shahsafi & Arian Rahimi · Microbiology Research Journal International · 2019

Backgrounds: As zoonotic infectious agents, Campylobacter spp. are important factors causing gastroenteritis in humans. Surveys show that the three strains; Campylobacter jejuni, Campylobacter coli and Campylobacter fetus play a major role in human infections. Identification of t...

Open access Research Article 10.9734/mrji/2019/v27i230095

Carbapenemase-Encoding Genes in Pseudomonas aeruginosa: Multiplex PCR Detection, Microarray Based Detection and Gene Sequencing

Ayman K. El Essawy, Hala M. Abu Shady & Bassem M. El Baiaa · Microbiology Research Journal International · 2018

Background: In 2017 the World Health Organization (WHO) classified carbapenem-resistant Pseudomonas among the most critical multidrug-resistant bacteria for urgent attention.   Aims: Evaluation of two molecular methods for detection of carbapenemase encoding genes in carbapenem r...

Open access Research Article 10.9734/MRJI/2018/42911

Study of Candida Species in Stool of Children with Autism Spectrum Disorders in Alexandria, Egypt

Shwikar Abdel Salam Ahmed, Marwa Ahmed Meheissen, Hanan Galal Azouz, Mona Hamdy Ashry, Yara Safwat Roshdy, Hala Abdelaty Gad & Ahmed Elsayed Ibrahim · Microbiology Research Journal International · 2017

Background and Aims: The pathogenesis of Autism spectrum disorders (ASDs) is still controversial. The aim of the present study was to investigate the presence and to identify the species and the antimicrobial sensitivity of Candida present in stool of autistic children. Subjects...

Open access Research Article 10.9734/MRJI/2017/32117

Transgenic Investigation of Canola (Brassica napus L.)

Sultan Fidan & Ercan Arican · Annual Research & Review in Biology · 2014

Investigating transgenic characteristics of Californium, Jura, Elvis and Orkan varieties of canola has been carried out in this study. These plants are widely cultivated in Turkey. The Canola varieties were grown in an established tissue culture and the total DNA isolated. The 35...

Open access Research Article 10.9734/ARRB/2014/11684