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PCR

Papers (38)

Clinical and Physiological Characterization of Canine Distemper Virus Infection in Dogs from the Junagadh Region of Gujarat, India

Jagruti N. Parmar, Rahul A. Chauhan, Devangini K. Pandya, Suresh V. Mavadiya, Arshi A. Vagh & Avinash K. Bilwal · Microbiology Research Journal International · 2025

Canine distemper virus (CDV) is an infectious disease of canines in the overall world, mainly in developing countries where proper immunization is not enclosed. This study was conducted to evaluate the clinical signs and physiological parameters alterations in canine distemper vi...

Open access Research Article 10.9734/mrji/2025/v35i121667

Assessment of the Association between Gross and Histopathological Lesions of Chronic Respiratory Disease and Molecular Detection of Mycoplasma gallisepticum by PCR in Chicken

Sharath Kumar B, Ravikumar P, Girish B.C, Shivakumar M.C, Naveen Kumar G.S, Sheela P, Shilpa V.T & Satheesha S.P · Microbiology Research Journal International · 2025

Aim: To study the gross & histopathological lesions in suspected cases of chronic respiratory disease and to correlate the gross chronic respiratory disease (CRD) lesions & molecular detection of Mycoplasma gallisepticum by polymerase chain reaction (PCR). Methodology: Th...

Open access Research Article 10.9734/mrji/2025/v35i41559

Prevalence of Sexually Transmitted Chlamydia trachomatis and Neisseria gonorrhoeae Infections in an Asymptomatic Population in Dakar, Senegal

Babacar Ndiaye, Abdoulaye Seck, Abdou Diop, Assane Dieng, Thierno Abdoulaye Diallo, Chantal Mahou & Philippe Dubrous · Microbiology Research Journal International · 2022

Introduction: Sexually transmitted infections (STIs) represent a major public health problem. Chlamydia trachomatis and Neisseria gonorrhoeae infections are often asymptomatic, thus leading to a high risk of transmission in subjects with risky behaviors. The objective of this stu...

Open access Research Article 10.9734/mrji/2022/v32i730398

Isolation, Identification and Characterization of Potential Probiotics from Fermented Food Products

Adenike O. Wejinya, Sunday Y. Giami, Lucretia I. Barber & Patience C. Obinna-Echem · Asian Food Science Journal · 2022

Potential probiotics were isolated, identified and characterised from ogi, fufu, nunu, palmwine and fermented tigernut milk. Pour plate method was used for the isolation of lactic acid bacteria (LAB) on De Man Rogosa Sharpe (MRS) media. Three (3) pure colonies were distinctly obt...

Open access Research Article 10.9734/afsj/2022/v21i530427

Role of PCR in Rapid Detection Group B Streptococcus in Pregnant Females in Al-Quwayiyah General Hospital, Riyadh, Saudi Arabia

E. S. Khater & A. S. Abdel-Motaal · Microbiology Research Journal International · 2021

Background: Group B streptococcus is one of the most common causes of severe neonatal infections. Aim: To detect the prevalence of group B. Streptococcus and their antimicrobial susceptibility and to assess the role of PCR as a rapid method of its detection. Place and Duration of...

Open access Research Article 10.9734/mrji/2021/v31i330305

Identification of Clostridium septicum in Clinical Cases Suspected to Braxy and Malignant Edema by Traditional and Molecular Method and Evaluation of Toxigenic Isolates for Vaccine Production

Lida Abdolmohammadi Khiav & Alireza Paradise · Microbiology Research Journal International · 2020

Clostridium septicum is the causative agent of the acute fetal disease braxy and gas gangrene with major economic losses in the dairy industry. Accurate and rapid detection is great importance in this regard. Vaccination is an effective process for protection against C. septicum...

Open access Research Article 10.9734/mrji/2020/v30i1230286

High Prevalence of Antibiotic Resistant Escherichia coli Serotype O157: H7 among Pastoral Communities in Rural Uganda

Abdul Walusansa, Jacob S. Iramiot, Christine F. Najjuka, Dickson Aruhomukama, Hussein Kafeero Mukasa, Henry Kajumbula & Benon B. Asiimwe · Microbiology Research Journal International · 2020

Background: Non-prescribed use of antimicrobials in Agriculture incurs a transfer risk of resistant pathogens to humans, complicating treatment. The aim of this study was to determine the potential of Zoonotic E. coli to serve as drivers of antimicrobial resistance (AMR) among an...

Open access Research Article 10.9734/mrji/2020/v30i630230

Optimization of Soil DNA Extraction Protocol Using Na2EDTA, SDS, Heating, Vortexing and CaCl2 and Its Validation for Metagenomic Studies

Malik Ahmed Pasha, Sachin A. More & P. U. Krishnaraj · International Journal of Environment and Climate Change · 2020

Aim: Several methods described previously for isolation and purification of soil DNA. Most of these protocols use combination of techniques or methods but the role and contribution of each individual method or component used is not clearly discussed. This study aims at analysing...

Open access Research Article 10.9734/ijecc/2020/v10i630201

Prevalence of Fusarium Species Associated with Peach Decline in Tunisian Nurseries

Sabrine Mannai, Najwa Benfradj, Najet Horrigue-Raouani & Naima Boughalleb-M’Hamdi · Microbiology Research Journal International · 2018

Aims: To survey nurseries and diagnosis of the young peach seedlings decline, to investigate the Fusariumspecies associated with the decline of peach in Tunisian nurseries  using morphological and molecular tools and determine the pathogenicity of the most predominant species on...

Open access Research Article 10.9734/MRJI/2018/40746

Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity

Zannati Ferdous Zaoti, S. M. Zia Hasan, Md. Firose Hossain, Md. Faruk Hasan, Md. Asadul Islam, Md. Khalekuzzaman & Biswanath Sikdar · Microbiology Research Journal International · 2018

Aims: Isolation and characterization of bacteria from the bacterial wilt disease of banana and evaluation of their sensitivity. Study Design:  This study was an experimental laboratory design. Place and Duration of Study: Disease infected banana plant leaves were collected from R...

Open access Research Article 10.9734/MRJI/2017/38206

Detection of Sutterella in the Stool of Egyptian Children with Autism Spectrum Disorders

Nancy Younis Omar, Shwikar M. Abdelsalam Ahmed, Hanan Azouz, Sarah Magdy Abdelhamid & Naglaa Moustafa Abdelaziz · Microbiology Research Journal International · 2017

Background: Autism spectrum disorder (ASD) is a multifaceted group of neurodevelopmental disorders. Gastrointestinal problems are commonly reported in children with autism and may correlate with autism severity. A recent study reported that Sutterella species were frequently foun...

Open access Research Article 10.9734/MRJI/2017/37988

Detection and Molecular Characterization of ESBLs in E. coli Isolates from a Tertiary Care Hospital in North India with Special Attention to CTX-M-27

Nitika Anand, Ashish K. Asthana & Molly Madan · Microbiology Research Journal International · 2016

Background: Extended spectrum beta-lactamases are the main cause of resistance to beta lactam antibiotics in members of Enterobacteriaceae. ESBL associated infections are on a rise worldwide and have become a serious public health problem. We aimed to investigate the molecular ep...

Open access Research Article 10.9734/BMRJ/2016/27860

Incidence of Heat-Labile Toxin (LT) Producing Enterotoxigenic Escherichia coli Mediated Diarrheal Disease in Egyptian Children under 5 Years Presenting to Tanta University Hospital

M. A. Abd El-Wahab, M. A. Shams eldin & M. A. Naiem · Microbiology Research Journal International · 2016

Background: Enterotoxigenic E. coli (ETEC) is under recognized but an important cause of diarrhea in the developing countries. It is the most frequent bacterial cause of diarrhea in children and adults living in these areas but is most frequently seen in children. We aimed to det...

Open access Research Article 10.9734/BMRJ/2016/26138

Molecular Characterization and Detection of Infection in Vector Snails of Urinary Schistosomiasis around Erinle and Eko Ende Dams in South West Nigeria

A. O. Hassan, A. O. J. Amoo, O. P. Akinwale, M. A. Adeleke & P. V. Gyang · Microbiology Research Journal International · 2016

Aims: The prevalence of the schistosome cercariae in snail intermediate hosts has been known as one of the valuable predictors of the level of schistosomiasis transmission in different localities. This study was undertaken to determine molecular characterization and detection of...

Open access Research Article 10.9734/BMRJ/2016/9019

Comparative Study between Polymerase Chain Reaction and Conventional Methods Used for Diagnosis of Clinically Suspected Onychomycosis

M. A. Abd El-Wahab, W. S. El Naghy & R. A. El Tatawy · Microbiology Research Journal International · 2016

Background: Proper treatment of onychomycosis depends mainly on accurate diagnosis. Diagnosis using conventional methods (direct microscopy and culture) is still insensitive and time consuming so we aimed to evaluate the commercially available PCR as a rapid method used for detec...

Open access Research Article 10.9734/BMRJ/2016/24852