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purification

Papers (13)

Study on the Purification and Reusability of Burnt Cooking Oil and Assessment of Its Physicochemical and Safety Parameters

B. M. Khaled, Adda Ann Sina, Asraful Alam, Md. Abdul Alim & Mrityunjoy Biswas · Asian Food Science Journal · 2023

Aims: The aim of the study was to investigate the reusability of edible oils and to find which temperature the refined oils could be used for cooking purposes. Study Design:  The study followed a completely randomized design (CRD). Place and Duration of Study: The study was condu...

Open access Research Article 10.9734/afsj/2023/v22i5633

Lipolytic Activities of Bacteria and Fungi Isolated from Soil Samples

Racheal Oluwayemisi Fashogbon, Bose Adebayo, Victoria Musa & Titilayo Femi-Ola · Microbiology Research Journal International · 2021

This study was carried out at the Department of Microbiology, Microbiology Laboratory, Ado-Ekiti State University, Ekiti State, Nigeria between July, 2018 to March, 2019. Due to the diverse biotechnological importance of lipases as a biocatalytic enzyme, extracellular production...

Open access Research Article 10.9734/mrji/2021/v31i530318

Characterization of Sesquiterpenes and Antibacterial Activities of Extracts from Piliostigma reticulatum (DL.) Hochst and Cleistopholis patens (Benth.) Engl & Diels against Shigella dysenteriae and Streptococcus pyogenes

A. O. Daniels, O. O. Owoyemi, O. Famurewa & J. O. Oluyege · Microbiology Research Journal International · 2019

Aim: The study characterized sesquiterpenes from the bark extracts of Piliostigma reticulatum and Cleistopholis patens and subsequently tested the extracts for their antibacterial activities. Methodology: Ground stem barks of P. reticulatum and C. patens were obtained and extract...

Open access Research Article 10.9734/mrji/2019/v27i530112

Biochemical Characteristics of Immobilized Chitinase from Alternaria infectoria

Hamed M. El-Shora, Salwa A. Khalaf & Suzan A. H. El-Sheshtawi · Microbiology Research Journal International · 2017

This research aimed to purify and immobilize chitinase from Alternaria infectoria and to study its biochemical characteristics for biotechnological applications. Chitinase (EC. 3. 2. 1. 14) was purified by ammonium sulphate precipitation, DEAE-cellulose and then Sephadex G-200 wi...

Open access Research Article 10.9734/MRJI/2017/36263

Production, Purification and Characterisation of a Purified Low Molecular Weight and Thermo-alkaline Tolerance Xylanase by Aspergillus brasiliensis

Hooi Ling Ho, Li Sha Soh & Soon Hwa Ong · Microbiology Research Journal International · 2015

Aims: Xylanase is an enzyme which has been used extensively in many different industrial processes. Xylanase also known as endo-1,4-β-xylanase is a glycosidase that catalyses the conversion of xylan to xylose through endohydrolysis of 1,4-β-D-xylosidic linkage which is commonly f...

Open access Research Article 10.9734/BMRJ/2016/20766

Production and Partial Purification of Cellulase from a New Isolate, Penicillium verruculosum BS3

Sreedharan Sajith, Sasidharan Sreedevi, Prakasan Priji, Kizhakkepowathial Nair Unni & Sailas Benjamin · Microbiology Research Journal International · 2015

Aims: This study describes the production of cellulases by a newly isolated fungus, Penicillium verruculosum BS3 on lignocellulosic biomass with special emphasis on endoglucanase. Study Design: Isolation, screening and characterization of cellulolytic fungus. Production of cellul...

Open access Research Article 10.9734/BMRJ/2015/17865

Purification, Characterization and Applications of Proteases Produced by Bacillus amyloliquefaciens 35s Isolated from Soil of the Nile Delta of Egypt

Fatma Refaat Nassar, Ahmed Abdelwahab Abdelhafez, Tarek Said El-Tayeb & Samah Hashem Abu-Hussein · Microbiology Research Journal International · 2015

Aims: The current study aimed at purifying and determining general characteristics of proteases produced by Bacillus amyloliquefaciens 35s isolated from the soil of Nile Delta of Egypt and to study its applications in environmental and industrial purposes. Study Design: The produ...

Open access Research Article 10.9734/BMRJ/2015/15504

Production, Purification and Characterization of Levan Polymer from Bacillus lentus V8 Strain

Khadiga A. Abou-taleb, Mohamed O. Abdel-Monem, Mohamed H. Yassin & Amal A. Draz · Microbiology Research Journal International · 2014

Aims: To optimize fermentation conditions for microbial production of levan polymer on a low-price productive medium. Also to purified levan from Bacillus sp. V8 strain, characterization the levan polymer by 13C NMR spectroscopy and physicochemical properties. Study Design: Optim...

Open access Research Article 10.9734/BMRJ/2015/12448

Purification of Paraoxonase (PON) from Sun Flower (Helianthus annuus) and Effects of Some Chemicals on Paraoxonase Activity In vitro

Nazan Demir, Hayrunnisa Nadaroglu & Yasar Demir · Annual Research & Review in Biology · 2014

Aims: In this study, paraoxonase (PON) enzyme was purified from mature seeds of sun flower by using affinity chromatography (Sepharose-4B-L-tyrosine-1-naphthylamine) and the effects of some chemicals were tested on paraoxonase activity as in vitro. Methodology: Paraoxonase was fi...

Open access Research Article 10.9734/ARRB/2015/10437

Purification, Biochemical Characterization and Applications of Pleurotus ostreatus ARC280 Laccase

Abdelmageed M. Othman, Ali M. Elshafei, Mohamed M. Hassan, Bakry M. Haroun, Maysa A. Elsayed & Ayman A. Farrag · Microbiology Research Journal International · 2014

Aims: To purify, characterize, and apply the laccase produced by submerged fermentation using an edible mushroom Pleurotus ostreatus ARC280. Study Design:  Laccase purification and characterization were designed using the most recent approaches and statistical studies of triplica...

Open access Research Article 10.9734/BMRJ/2014/11218

Kinetic Properties of Purified β-Mannanase from Penicillium italicum

O. O. Olaniyi, D. J. Arotupin, B. J. Akinyele & O. S. Bamidele · Microbiology Research Journal International · 2014

Aim: The study aimed at purification and characterization of β-mannanase from Penicillium italicum. Study Design: The first experiment, β-mannanase from Penicillium italicum was produced in basal medium supplemented with Locust Bean Gum (LBG). The second described the purificatio...

Open access Research Article 10.9734/BMRJ/2014/6555

Purification, Kinetic Properties and Antitumor Activity of L-Glutaminase from Penicillium brevicompactum NRC 829

Ali Mohamed Elshafei, Mohamed Mohamed Hassan1, Nadia Hussein Ali, Mohamed Abd-Elmontasr Abouzeid, Dalia Ali Mahmoud & Dina Helmy Elghonemy · Microbiology Research Journal International · 2013

Aim: The aims of the present study were to purify and characterize L-glutaminase from Penicillium brevicompactum NRC 829; and to evaluate the antitumor activity of the purified enzyme against different tumor human cell lines. Study Design: Testing of antitumor activity of L-gluta...

Open access Research Article 10.9734/BMRJ/2014/5098

Purification, Characterization and Antitumor Activity of L-asparaginase from Penicillium brevicompactum NRC 829

Ali Mohamed Elshafei, Mohamed Mohamed Hassan, Mohamed Abd-Elmontasr Abouzeid, Dalia Ali Mahmoud & Dina Helmy Elghonemy · Microbiology Research Journal International · 2012

Aim: The aims of this study were to attempt to extract, purify and characterize of L-asparaginase, an antitumor agent, from Penicillium brevicompactum NRC 829. Study Design: Testing of antitumor activity of L-asparaginase against four different tumor human cell lines. Place and D...

Open access Research Article 10.9734/BMRJ/2012/1735