Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil
Andressa Sbano, Juliana B. Succar, João Victor R. Ferreira, Lidiane C. Berbert, Vinicius R. Flores, Janaína J. V. Cavalcante, Rodolpho M. Albano, Ida Carolina N. Direito, Alexander M. Cardoso
Asian Journal of Biotechnology and Bioresource Technology · pp. 11–13 · Published 12 Feb 2020
10.9734/ajb2t/2020/v6i130071Abstract
Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil. Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019. Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST). Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs.
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