Isolation, Production and Purification of Antimicrobial Peptides from Microbial Species Isolated from Manure and Banana Farm Soil

Abstract

Aim: This study focused on isolating and purifying Antimicrobial peptides (AMPs) from soil bacteria collected from manure and banana farm soil samples. Antimicrobial peptides (AMPs) are natural bactericidal compounds produced by a variety of organisms, including microorganisms and plants. Study Design: Isolation of Soil bacteria was done from Manure soil, Peepal tree, and Banana Farm & were screened for AMP isolate. Production and Purification of Antimicrobial peptides (AMPs) was carried out and were studied for their antimicrobial activity Place and Duration: Dr. D. Y. Patil, Arts, Commerce & Science, College, Pimpri, Pune- 18, December 2022 to March 2023. Methodology: The bacteria were cultured on nutrient agar plates, and the antimicrobial peptide producers were identified using the Agar overlay assay method, where clear zones of inhibition indicated the presence of antimicrobial-producing bacteria. Isolates with significant antimicrobial peptide producing potential, which inhibited the growth of sensitive strains in applied assay proceed for bulk production. After production antimicrobial peptides were purified by chloroform. Result: Antimicrobial activity of partially purified antimicrobial peptides was checked against Escherichia coli and Staphylococcus aureus. Maximum antimicrobial activity was found against Staphylococcus aureus on MH agar, with an inhibitory zone of 17 mm, increasing to 25 mm after partial purification with chloroform. Conclusion: This study indicates that valuable antibacterial peptide compounds can be derived from unexplored ecological niches. Antimicrobial peptides are increasingly recognized as promising alternatives for clinical therapies and as preservatives in the food industry, helping to prevent food spoilage. Their appeal lies in their broader antimicrobial spectrum, particularly effective against closely related bacteria.