Antioxidant Activities of Vitamin C and Bi-carbonate Buffers on Hormones Secretion and Serum Metabolites of Heat-stressed Rabbit Buck

Abstract

Aims: To evaluate the antioxidant activities of vitamin C and bicarbonate buffers on hormones secretion and serum metabolites of heat-stressed rabbit buck. Methodology: A total of twenty-five (25) Adult rabbit bucks (New Zealand White crosses) of 12 months old were used in this study. The rabbits were randomly allotted into the experimental treatments of five treatment groups with five (5) rabbits per treatment in a Completely Randomized Design (CRD). The treatment groups consisted of Control, Sodium Bicarbonate (Na2HCO3) and Potassium bicarbonate (KNO3), Vitamin C, and baobab fruit pulp meal (BFPM) as supplements respectively. The experiment lasted for 9 weeks. Temperature humidity of the pen house and thermoregulatory condition of the rabbits were evaluated. Blood samples (5 ml) were collected from the ear vein at 10.00 h from four rabbits chosen randomly from each group of rabbits respectively before and the end of the experiment for serum metabolite, thyroxine and testosterone hormones evaluation. Results: It was found that Vitamin C and BFPM significantly (P<0.05) reduced thermoregulatory parameters and increased hormonal secretions. The pattern of secretion of serum metabolites was not significantly (P<0.05) consistent. Conclusion: It was concluded that Vitamin antioxidants were more effective to ameliorate heat stress than bicarbonate buffers.