Interferon-γ, Interleukin 1-β and Tumor Necrosis Factor-α levels and Their Association with Lipid and Glycaemic Profiles in Diabetic Mice

Abstract

The cytokines IFN-γ, interleukin IL-1β, and TNF-α each up regulate the expression of major histocompatibility complex (MHC) and are therefore considered as inflammatory markers. The present study aimed to measure the serum levels of IFN-γ, interleukin IL-1β, and TNF-α in mice after induction of diabetes with streptozotocin and to correlate their levels to lipid and glycaemic profiles. The study included 40 Swiss Albino mice (20 males and 20 females), half of each male and female groups were made diabetic by intraperitoneal injection of streptozotocin. After 48 hours, the serum levels of INF-γ, IL1-β as well as TNF-α were measured with ELISA and their levels were correlated to lipid and glycaemic profiles. The levels of the cytokines, IFN-γ, IL1-β and TNF-α were measured and correlated to lipid profile, blood glucose and insulin. The serum levels of the three studied cytokines, IFN-γ, IL 1-β and TNF-α were statistically significantly higher among diabetic mice compared to the control group. Diabetic male mice (M-STZ mice) group showed significantly higher lipid profile compared to the control group (M-control). Cholesterol level was significantly higher among female control (F-control) compared to the M-control group. Cholesterol level was significantly higher among diabetic female mice (F-STZ mice) compared to the F-Control group. Regarding IFN-γ, IL 1-β and TNF-α levels, there were significant linear correlations with the glycemic profile (Glucose, insulin) reflected as positive correlation with blood glucose level and negative correlation with insulin level.