Improvement of Antibacterial and Wound Healing Properties of Resorbable Biomaterials Using 1-(Alkylamino) Isoquinoline Derivatives

Abstract

One of the problems in implantation of resorbable materials into the body is inflammation and purulent processes after surgery caused by pathogenic bacteria. There is a lot of information in the literature on the study and elimination of this problem. In order to expand the possibility of using resorbable materials, it is important to search for new drugs with antibacterial and wound-healing properties. In the article are presented the results of research on obtaining materials with antibacterial and wound healing properties by incorporating 1-(alkylamino) isoquinoline derivatives into PLA/HA-based resorbable biomaterials. It was found that the antibacterial properties increase with the increasing of the alkyl group from methyl to butyl in 1C of 1-(alkylamino) isoquinoline derivatives. According to the results of molecular docking to the enzymes 6COX (cyclooxygenase-2) and 3V99 (lipoxygenase-5), synthesized derivatives can occur binds between ligands and protein molecules hydrogen and hydrophobic bonds, as well as π–π stacking interactions. It was shown that the release of drugs from their contents in an aqueous environment, included in the entire volume of the material, reaches its maximum value in 8-10 days. The synthesized materials have the properties of healing artificial wounds in vitro in fibroblast cell line L929 cells, and in 72-hour experiments was healed about 90% of the wounds.