Effect of Different Plant Growth Regulators on In-vitro Micropropagation of Banana Cultivar Grand Naine (Musa spp.)

Abstract

Banana (Musa sapientum) is one of the most important fruit crop cultivated in India. It is widely grown in more than 150 countries, producing on an average of 105 million tons of fruit per annum. Grand naine Banana variety is the most commonly cultivated banana with high yielding plantain. This variety mainly grown in the region of Uttar Pradesh state. Present study mainly focuses on the in-vitro micropropagation method which is described for banana cv. Grand naine. For this study, healthy sword suckers of three months old banana plantlets was used as explants. The explants was sterilized thoroughly and cultured on MS (Murashige and Skoog) media supplemented with the different concentration and combination of auxin and cytokinin like BAP, IAA and NAA which were used for the optimization of best plant growth regulator combination for multiple shoot generation and shoot growth. Among all the tested cytokinin 5 mg/l BAP along with 1mg/l IAA in medium concentration performed well and produced healthy shoots further at higher concentration of all tested cytokinin showed declining effect within the growth parameters. For the rooting purpose, MS media was supplemented with different levels of IBA, IAA and NAA. Results indicated that rooting was highly promoted in the medium supplemented with 0.5 mg/L IAA + 1.5 mg/L IBA.