Evaluation of Genetic Variability in  Adansonia digitata L. by RAPD Analysis

Laxmi Gautam; Showkat Ahmad Ganie; Rajneesh Kumar Agnihotri; Rajendra Sharma

doi:10.9734/BBJ/2015/18727

Research Article Open access CC BY 4.0

Evaluation of Genetic Variability in Adansonia digitata L. by RAPD Analysis

Laxmi Gautam, Showkat Ahmad Ganie, Rajneesh Kumar Agnihotri, Rajendra Sharma

Biotechnology Journal International · pp. 1–6 · Published 19 Aug 2015

10.9734/BBJ/2015/18727

Abstract

Baobab (Adansonia digitata L.) belonging to Bombacaceae family, is one of the most widely used indigenous tree species in sub-Saharan Africa. The objective of the present study was to assess molecular variation among A. digitata and to determine the level of genetic similarity among them. The yield of DNA ranged from 15-40 µg/mg of leaf tissue and the purity was between 1.1- 2.9, indicating minimal levels of contaminating metabolites. The technique was ideal for isolation of DNA from A. digitata and the DNA isolated was used for randomly amplified polymorphic DNA (RAPD) analysis using the primer OPB07. The bands obtained ranged in size from 54-795 bp. Two clusters were observed, one group with 8 bands and the other with 11 bands. Present study could be important in domestication, conservation, management & improvement strategies of A. digitata.

Amplification Baobab conservation genetic diversity RAPD

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Laxmi Gautam, Showkat Ahmad Ganie, Rajneesh Kumar Agnihotri, Rajendra Sharma (2015). Evaluation of Genetic Variability in Adansonia digitata L. by RAPD Analysis. Biotechnology Journal International , 1–6. https://doi.org/10.9734/BBJ/2015/18727

Affiliations

Laxmi Gautam — Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India
Showkat Ahmad Ganie — Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India
Rajneesh Kumar Agnihotri — Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India
Rajendra Sharma — Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India

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