Inhibitory Effects of Azadirachta indica (Neem) and Bryophyllum pinnatum (Oda Opue) Leaves Extracts on Staphylococcus aureus Isolated from Infected Wound Samples
M. I. Izundu, C. O. Anyamene, E. A. Kyrian- Ogbonna, O. R. Umeh, I. U. Nwiyi
Journal of Advances in Medical and Pharmaceutical Sciences · pp. 18–26 · Published 21 Aug 2021
10.9734/jamps/2021/v23i630241Abstract
Aim: to study the antimicrobial effects of different leaves extracts of Azadirachta indica and Bryophyllum pinnatum on Staphylococcus aureus isolated from infected wound. Study Design: This study was done to determine inhibitory effects of extracts on S. aureus isolated from infected wounds. Place and Duration of Study: General hospital Onitsha, Anambra State, Nigeria, January to March 2020. Methodology: wound swabs were collected from 30 patients with infected wound. S. aureus were isolated and identified morphologically and biochemically. The leaves were collected, identified and grinded. Extraction of the leaves were done using soxhlet method with two solvents (ethanol and water). Phytochemical compositions were analyzed using standard methods. In vitro antimicrobial effect of extracts and their combination were evaluated. Results: Phytochemical analysis of extracts showed presence of saponins, flavonoids, steroids, alkaloids, tannins, cardiac glycosides and phenols except B. pinnatum aqueous extract that showed presence of flavonoids, saponins and alkaloids. Minimum inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) were determined. Among the extracts analysed, A. indica ethanol has highest zone of inhibition against S. aureus with 20.00±0.00 mm at 1000 mg/ml concentration. B. pinnatum ethanol 10.33±0.58 mm at 1000 mg/ml, A. indica aqueous extract 13.33±2.08 mm but B. pinnatum aqueous extract has no inhibitory effect. The combination of A. indica aqueous and B. pinnatum ethanol extracts, B. pinnatum and A. indica ethanol extracts and A. indica ethanol extract and B. pinnatum aqueous extracts have inhibitory effects on S. aureus except combination of A. indica aqueous and B. pinnatum aqueous which has no inhibitory effect. The result of MIC was 500 mg/ml and the MBC showed 1000 mg/ml. Conclusion: The extracts of B. pinnatum and A. indica possess antimicrobial properties for S. aureus and the plants are available and are planted worldwide. There is also need for research on other plants with antimicrobial effects.
Cited by 0
No indexed citations yet.
Related research
- Ameliorative Histological Effects of Aqueous Neem Leaf Extract (Azadirachta indica) on Lead Induced Neurotoxicity of Lateral Geniculate Body of Adult Wistar Rats — shares topic coverage
- Antifungal Activity of Various Plant Extracts against Aspergillus and Penicillium Species Isolated from Leather-Borne Fungus — shares topic coverage
- Evaluation of Tyrosinase Producing Endophytic Fungi from Calotropis gigantea, Azadirachta indica, Ocimum tenuiflorum and Lantana camara — shares topic coverage
- Enhancing Soil Fertility Status, Sweet Potato Yield and Tuber Nutrient Composition through Different Manure Sources in Southeastern Nigeria — shares topic coverage
- Reversibility, Body Weight and Histo-architectural Alterations in the Anterior Pituitary Gland of Aqueous Azadirachta indica Extract Fed Wistar Rats — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.