A New Centrifugation Method for the Improvement of Platelet-rich Fibrin Products: A Preliminary Study

Abstract

Introduction: Nowadays, there are many articles about Platelet Rich Plasma/Platelet Rich Fibrin families. A novel platelet-rich product called titanium prepared platelet-rich fibrin (T-PRF) has stronger and thicker fibrin than that of the classic glass tube prepared platelet-rich fibrin. Strong fibrin structure is important to extend the time for resorption of fibrin in-vivo, and increase the release time of growth factors. Objective: In this preliminary study of a new centrifugation method, we aimed to change the direction of fibrin formation during the platelet aggregation, and make T-PRF much denser and more resistant. According to our hypothesis, it can make it possible to use in guided bone, and guided tissue regeneration more successfully. Methods: Blood samples of 10 healthy male volunteers were collected, and four 10ml blood samples, one for each of four groups, were transferred to a Ti tube from each volunteer. The first group was centrifuged for a 20-minute period clockwise (T-PRF group), and the other groups were centrifuged for a total of 20 minutes with two-minute (2min MT-PRF group), five-minute (5 min MT-PRF group), and ten-minute (10min MT-PRF group) periods clockwise and counter-clockwise. Results: By hematoxylin and eosin stain, the 10min MT-PRF group showed a better-organized network with continuous integrity compared to the other groups. With the immunofluorescent staining, fibrin seemed thicker and better organized in the 10 min MT-PRF group. SEM examination showed more complex and denser fibrin clusters in the 10 min MT-PRF group than the other groups. Conclusion: This pilot study defines 10 min MT-PRF as a new autogenous product with superior fibrin network. Our results showed that, fibrin formation was made more organised and denser with 2-way direction centrifugation.