In vitro Salinity Evaluation Studies in Golden Berry (Physalis peruviana L.)
Fatma Burcu Celikli, Pınar Akkelle, Ahmet Naci Onus
Biotechnology Journal International · pp. 1–8 · Published 8 Nov 2017
10.9734/BJI/2017/37097Abstract
Aim: Present study, therefore, was conducted in in vitro conditions to study effect of NaCl at varied levels on growth and chlorophyll content of golden berry shoot apices grown in in vitro conditions. Place and Duration: The study was carried out in the Department of Horticulture, Faculty of Agriculture, Akdeniz University, Antalya, Turkey, between March-June, 2017. Methodology: In this study in vitro salinity of golden berry shoot apex culture were studied within the Murashige and Skoog nutrient medium with 1 mg L-1 indole acetic acid (IAA)+ 3% sucrose and 0.7% agar supplemented with NaCl (0; 25; 50; 75 and 100 mM). The explants were incubated at 25±2°C for 4 weeks and related parameters, such as shoot, leaf and root formation, were measured. Results: Experimental results revealed that different level of salinity treatments in in vitro culture had notable effect on above stated growth parameters. These parameters decreased significantly by increasing salinity level for excluding shoot diameters.
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