Advanced Platelet- Rich Fibrin (A-PRF): A Gender Based Study on Cell Population

Abstract

Platelet Rich Fibrin (PRF) is a natural fibrin-based biomaterial prepared from an anticoagulant-free blood harvest without any artificial biochemical modification that allows obtaining fibrin membranes enriched with platelets and growth factors. PRF is superior to other platelet concentrates like Platelet Rich Plasma (PRP) due to its ease and inexpensive method of preparation and also it does not need any addition of exogenous compounds like bovine thrombin and calcium chloride. A blood sample of 10 individuals with healthy periodontium and no systemic diseases was collected and centrifuged to get platelet rich fibrin. Platelet rich fibrin is studied histologically to compare the ability of tissue regeneration and wound healing capacity between males and females. Neutrophils and lymphocytes are increased in A-PRF compared to S-PRF. Neutrophils and lymphocytes are proportionately more in males than females in both A-PRF and S-PRF. Monocytes are similar in A-PRF & S-PRF. Cells are more concentrated in S-PRF. Cells are more widely distributed in A-PRF. Fibrin is more dense in A-PRF compared to S-PRF. Hence A-PRF especially in males could have a greater potential of tissue regeneration and wound healing capacity. Although it is not conclusive due to limited sample size, further increase in sample size can throw more light on the clinical implications. The difference in regenerative potential of platelet concentrates can pave the way for specialised and focussed treatment strategies resulting in more favourable patient outcomes.