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Androgenic Properties and Subchronic Toxicity of the Aqueous Extract of Pycnanthus angolensis (Welw.) Warb. Wood (Myristicaceae)

Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Yannick Sani Jignoua, Alfloditte Flore Feudjio, Arnaud Choupo, Fabiola Jenny Massah, Constant Anatole Pieme, Paul Fewou Moundipa

Journal of Complementary and Alternative Medical Research · pp. 34–45 · Published 2 Apr 2024

10.9734/jocamr/2024/v25i3526

Abstract

Aims: Pycnanthus angolensis (Welw.) Warb. also known as “Etengué” in the Baka language, is a medicinal plant used by the Baka Pygmies of Cameroon to treat erectile dysfunction. This study aimed to evaluate the androgenic activity and subchronic toxicity of the aqueous extract of Pycnanthus angolensis wood. Study Design:  Experimental design. Place and Duration of Study: Laboratory of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, between November 2022 and October 2023. Methodology: Twenty-five male Wistar strain rats were divided into 5 groups of 5 rats each including a positive control group that received testosterone enanthate (5 mg/kg) intramuscularly once a week; a normal control group that received distilled water (10 ml/kg) and three test groups which received 134 and 267 and 533 mg/kg of the aqueous extract of Pycnanthus angolensis daily per os. On the 29th day, the animals were killed, and several biochemical parameters were assessed for androgenic properties. Results: At a dose of 134 mg/kg, the extract significantly increased (p<0.05) the levels of testicular cholesterol, testosterone and serum prostatic acid phosphatase activity compared to those of the normal control. A toxicity study showed that at doses of 134 and 267 mg/kg, the extract did not induce any significant variation (p>0.05) in the creatinine level, but a significant reduction (p<0.05) in of aspartate and alanine aminotransferase activities was recorded. Histopathology revealed destruction of testicular spermatozoa at a dose of 533 mg/kg compared to that of the controls. Conclusion: These results showed that at a dose of 134 mg/kg, the aqueous extract of Pycnanthus angolensis (Welw.) Warb. wood is capable of stimulating the production of androgens and is tolerated by the rat's body. Our results justified the traditional use of this plant for the treatment of male infertility and erectile dysfunction.

Pycnanthus angolensis androgenic properties subchronic toxicity male rat

References (30)

  1. 1 Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative Ultracentrifuge [DOI]
  2. 2 DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION [DOI]
  3. 3 A Colorimetric Method for the Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminases [DOI]
  4. 4 Enzymatic Determination of Total Serum Cholesterol [DOI]
  5. 5 Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. [DOI]
  6. 6 Serum kreatininbestimmung ohne enteiweissen [DOI]
  7. 7 Membrane Interactions of Phytochemicals as Their Molecular Mechanism Applicable to the Discovery of Drug Leads from Plants [DOI]
  8. 8 National Cholesterol Education Program recommendations for measurement of high-density lipoprotein cholesterol: executive summary. The National Cholesterol Education Program Working Group on Lipoprotein Measurement [DOI]
  9. 9 Physiology of Testicular Function [DOI]
  10. 10 Evaluation of androgenic activity of Zingiber officinale and Pentadiplandra brazzeana in male rats.
  11. 11 The Relationship between Testosterone Deficiency and Men's Health [DOI]
  12. 12 Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents [DOI]
  13. 13 Androgenic potentials of aqueous extract of Massularia acuminata (G. Don) Bullock ex Hoyl. stem in male Wistar rats [DOI]
  14. 14 Anticancer and antioxidant activities of methanol extracts and fractions of some Cameroonian medicinal plants [DOI]
  15. 15 In vitro screening of the potential of six Cameroonian medicinal plants on male reproductive biomarkers [DOI]
  16. 16 Classification of Andrological Disorders [DOI]
  17. 17 Enquete sur les aspects toxicologiques de la phytotherapie utilisee par un herboriste à Fes, Maroc [DOI]
  18. 18 Modulatory effects of artichoke ( Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats [DOI]
  19. 19 Anabolic and androgenic activities of Bulbine natalensis stem in male Wistar rats [DOI]
  20. 20 Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model [DOI]
  21. 21 Androgenic effects of aqueous and methanolic extracts of Ficus asperifolia in male Wistar rats [DOI]
  22. 22 Acute and 28‐Day Repeated‐Dose Oral Toxicity Assessment of Abelmoschus moschatus Medik. in Healthy Wistar Rats [DOI]
  23. 23 Acute and subacute toxicity of the aqueous extract of Amaranthus viridis (Amaranthaceae) leaves in rats [DOI]
  24. 24 Antimicrobial properties and acute toxicity evaluation of Pycnanthus angolensis stem bark [DOI]
  25. 25 Potential of Leaves of Water Mimosa (Neptunia plena) in Increasing Spermatogenesis Activity of Male Rat [DOI]
  26. 26 Effects of the aqueous extract of Pycnanthus angolensis (Myristicaceae) on male rat sexual behavior and nitric oxide release [DOI]
  27. 27 Non-clinical toxicity studies on bioactive compounds within the framework of nutritional and health claims [DOI]
  28. 28 The Conundrum of the Efficacy of Tribulus terrestris Extract as Supplement for Muscle Mass Gain [DOI]
  29. 29 Antioxidant potential of some Nigerian herbal recipes [DOI]
  30. 30 Evaluation of the Androgenic Properties of the Aqueous Extract of the Trunk Bark of Spathodea Campanulata P. Beauv. (Bignoniaceae) in Male Rats [DOI]

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