Antibacterial Activity of Ananas comosus Fruit Extract against Clinically Isolated Bacteria from Urinary Tract Infected Patients

Abstract

Aim: To evaluate the antibacterial activity of Ananas comosus (A. comosus) fruit extract against clinically isolated bacteria from urinary tract infected patients. Study Design: Experimental study. Place and Duration of Study: Research Lab, Department of Siddha Medicine, Tamil University, Thanjavur, India and Microbiology lab, School of Pharmacy, KPJ Healthcare University College, Malaysia, between February 2019 and January 2020. Methodology: In the present study the ethanol and aqueous extracts of A. comosus were analysed for the phytoconstituents and the activity of the plant extract was compared with a standard antibiotic which is used for a wide range of Urinary Tract infection which is Ciprofloxacin (250 mg/mL) using cup plate method. Results: The ethanolic extract of A. comosus showed a great level of bacterial inhibition (27.3 mm) against Bacillus cereus as compared with standard (22.3 mm).Whereas the antimicrobial activity was moderate against Klebsiella organism and very less against Staphylococcus. Standard exhibits a huge difference in the zone of inhibition which is (34 mm) and ethanol extract (23 mm) against Enterococcus. While the aqueous extracts do not show any effect on the microorganism. Conclusion: The ethanol extract A. comosus exhibited broad-spectrum activity against tested isolates compared to aqueous extract. A. comosus has broad inhibitory activities to pathogenic microorganisms and promising to act as potential antibacterial agents from natural plant sources.