Antibiotic Susceptibility and Intestinal Epithelial Cell Adhesion Pattern of Homo Fermenting Lactic Acid Bacteria (LAB) Isolated from Kunu-Zaki, a Spontaneously Fermenting Nigerian Cereal Beverage
S. O. Oluwajoba, F. A. Akinyosoye, V. O. Oyetayo
Microbiology Research Journal International · pp. 1311–1324 · Published 20 Jul 2014
10.9734/BMRJ/2014/10772Abstract
Aim: To determine the antibiotic reaction and adhesion pattern of antimicrobial homo-fermenting LAB strains in the fermenting slurries of kunu zaki. Study Design: ANOVA. Inhibition of indicator lawn used ≥10mm inhibition as antibiotic susceptible. Adhesion was measured by staining and quantifying recorded as percentage and index values. Place and Duration of Study: Department of Microbiology, Federal University of Technology Akure and Biotechnology Unit, Federal Institute of Industrial Research, Oshodi, Nigeria between June, 2012 and December, 2012. Methodology: Kunu-zaki drinks were produced using spontaneously fermenting cereal grains of Digitaria exilis (acha), Sorghum bicolour (sorghum) and Pennisetum americanum (millet) in composite and non-composite proportions. LAB isolates were obtained on MRS agar. Homo-fermenting isolates were identified to species level using the API 50 CHL test kit. Antibiotic sensitivity testing on the identified isolates followed the modified standard Kirby-Bauer procedure on MRS agar (pH 7.4) using the disc diffusion technique with selected antibiotics. For quality control of the antibiotics, sensitive reference strains S. aureus ATCC 25923 and E. coli ATCC 25922 obtained from the Nigeria Institute of Medical Research were used. Adhesion and antimicrobial properties were determined using standard method. Results: Antimicrobial substances produced by the eight LAB isolates inhibited the growth of four selected human pathogens in vitro. All eight LAB isolates were resistant to amoxicillin, gentamycin and ciprofloxacin. L. plantarum126, L. paracasei subsp paracasei339 and Pediococcus damnosus32 were resistant to erythromycin whilst all others were susceptible. L. plantarum126 and L. paracasei subsp paracasei339 were resistant to all antibiotics tested. All LAB isolates demonstrated high in-vitro intestinal epithelial cell adhesion potential. Conclusion: LAB antimicrobial activity may not be affected if kunu zaki is consumed simultaneously with these antibiotic therapies. However, if these LAB strains are intended for use as kunu-zaki starter cultures, it is important that they should be further carefully examined for inability to transfer antibiotic resistance genes to food pathogens
References (23)
- 1 Lactic acid bacteria as functional starter cultures for the food fermentation industry [DOI]
- 2 Antibiotic resistance in food lactic acid bacteria—a review [DOI]
- 3 The Lactic Acid Bacteria: A Literature Survey [DOI]
- 4 Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract [DOI]
- 5 Acquired antibiotic resistance in lactic acid bacteria from food [DOI]
- 6 Selection of Probiotic Strains for Human Applications [DOI]
- 7 Microbiological characterization and probiotic potential of koko and koko sour water, African spontaneously fermented millet porridge and drink [DOI]
- 8 Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine [DOI]
- 9 Vancomycin resistance factor of Lactobacillus rhamnosus GG in relation to enterococcal vancomycin resistance (van) genes [DOI]
- 10 Antibiotic resistance spread in food [DOI]
- 11 The possibilities of potentiating the efficacy of probiotics [DOI]
- 12 Presence of drug resistance in intestinal lactobacilli of dairy and human origin in Turkey [DOI]
- 13 High levels of spontaneous drug resistance in Lactobacillus [DOI]
- 14 The microbiology of ?kunun-zaki?, a cereal beverage from northern Nigeria, during the fermentation (production) process [DOI]
- 15 Acquired antibiotic resistance in lactic acid bacteria from food [DOI]
- 16 Sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1 [DOI]
- 17 Plasmid profiles and antibiotic susceptibility patterns of Lactobacillus isolated from fermented foods in Nigeria [DOI]
- 18 ANTIMICROBIAL SUSCEPTIBILITY PATTERNS OF LAB ISOLATED FROM WAKALIM, A TRADITIONAL ETHIOPIAN FERMENTED SAUSAGE [DOI]
- 19 Effect of Processing on Nutritional, Microbiological and Sensory Properties of Kunun-Zaki (A Sorghum Based Non-Alcoholic Beverage) Widely Consumed in Nigeria [DOI]
- 20 Antimicrobial susceptibility pattern of lactic acid bacteria isolated from ergo , a traditional Ethiopian fermented milk, Jimma, south west Ethiopia
- 21 Study on the pH of ogi, Nigerian fermented weaning food, and its effect on enteropathogenic Escherichia coli, Salmonella typhi and Salmonella paratyphi.
- 22 Lactobacillus adhesion to epithelial cells from bovine vagina
- 23 Isolation and molecular characterization of lactic acid bacteria from cheese
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