Assessment of Bacterial Micro Colonies Present in Tissue Samples Obtained Using Ozonated Water as an Irrigating Agent from Patients with Periodontitis

Abstract

Periodontal diseases are chronic inflammatory diseases that are constantly associated with microorganism, conventionally scaling and root planing followed by flap surgical procedure is done to treat chronic periodontitis condition, irrigating agents are used during both sub gingival scaling and root planing procedure and in flap surgical procedure. Saline and distilled water are the commonly used irrigants and they non specifically reduce the bacterial count, Ozonised water is also used as an irrigating agent. Though it’s effect on plaque is known to be effective its effect on tissues is still questionable. The study is conducted to assess the number bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents. The aim of the study is to assess the bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents. A randomized, split mouth study was performed. A total of 10 patients suffering from chronic generalised periodontitis were recruited into the study; 20 gingival tissue samples were obtained from the patients at the time of surgery. Both the patient and the clinician were blinded regarding the type of the irrigating agent used. The plaque samples were transferred into carrier medium and were cultured for 24 hours; 24 h after culture, the samples were collected and total colony forming units were calculated. On  counting  the number of  colony forming  units formed, it  was  found   that  the  amount  of colonies formed  from the samples treated with  ozone  showed mixed results, wherein it showed both increase and decrease in colony counts.