Attenuating Effect of Fraction of Dryopteris dilatata on Alloxan-induced Oxidative Stress and Hepato-nephro Injury in Wistar Rat Models

Abstract

Aims: Prolonged hyperglycemia leads to diabetes mellitus, causes serious complications which is a major concern affecting global economy.  Dryopteris dilatata (Dd) leaf contains phytochemicals with capacity to treat metabolic disorders.  The present study was carried out to evaluate the protective effect of fractions of Dd on alloxan induced oxidative stress, hepatonephro injury in Wistar rat’s models. Methods: Thirty-six male wistar rats (135-140) g divided into six groups (n-6) were used for the study, diabetes was induced into thirty rats through intra-peritoneal injection of 100 mg/kg of alloxan. Group 1 served as normal control, groups 2-6 were induced with diabetes using alloxan (100 mg/kg); group 2 served as diabetic control, while groups 3-6 served as treatment groups and received Metformin 50 mg/kg, reducing sugar, alkaloid and tannin fractions 800 mg/kg of Dd respectively throughout the treatment duration (15 days). We evaluated lipid peroxidative activity, endogenous antioxidants on the pancreas, liver and kidney, liver and kidney function biomarker. Results: The results revealed the fractions of Dd significantly increased body weights, reduce fasting blood glucose levels of experimental animals, decreased malondialdehyde (MDA) levels and increased the levels of catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD). It also attenuated kidney and liver injury, in diabetic Wistar rats. Conclusion: The observation in this study indicates that fractions of Dd leaf possess anti-diabetic, anti-hepato-nephro activities through ameliorating endogenous antioxidant activity in hyperglycemic state.