In vitro Measurement of Anticoagulant Effect of Acacia Nilotica Aqueous Extract on Normal Human Plasma

Abstract

Aims: To determine the anticoagulant effect of Acacia Nilotica aqueous extract on normal human plasma. Study Design: This is an experimental study. Place and Duration of the Study: Department of Haematology, Faculty of Medical Laboratory sciences, Alzaiem Alazhari, Khartoum – Sudan, during the period from July – August 2021. Methodology: A total of 20 human blood samples were collected for this study from apparently healthy subjects following ethical considerations. Samples were collected in tri-sodium citrate and platelets poor plasma (PPP) were immediately prepared by centrifugation. Aqueous solution of Acacia nilotica was prepared with distilled water (D.W), three concentrations of the solution (50%, 75% and 100%) were prepared. Prothrombin Time (PT) and Activated partial thromboplastin time (APTT) were tested by manual method before and after adding different concentrations of Acacia Nilotica solution. Statistical tests were performed by using SPSS version 16. Results: The results of study showed that Acacia Nilotica significantly prolonged the PT and APTT results of human plasma (P values for 50%, 75% and 100% solutions  were 0.000, 0.000 and 0.000 while for APTT 0.036, 0.000 and 0.000 for PT respectively). Also the study showed no significant correlation between control and A. nilotica concentrations among coagulation profile, except a significant positive correlation between control and 75% Acacia nilotica on APPT. Conclusion: Acacia Nilotica solutions had anticoagulant effect in human plasma, and could be nominated as a potential preventive agent for thromboembolic diseases.