Phytochemical Screening, Polyphenol Content and Antioxidant Studies of Ethanol Leaf Extract of Combretum aculeatum vent.
Alioune Dior Fall, Awa Ndiaye Sy, Jean Baptiste Hzounda Fokou, Joël Olivier Ndiefi Fomi, Mbaye Dieng, Serigne Ibra Mbacké Dieng, Emmanuel Bassene
European Journal of Medicinal Plants · pp. 1–7 · Published 1 Sep 2015
10.9734/EJMP/2015/20294Abstract
Objectives: The present study aimed to identify the phytochemical groups of ethanol leaf extract of Combretum aculeatum Vent., and to evaluate the phenol content of extracts and their antioxidant activity. Materials and Methods: Collected leaves were extracted with ethanol (95°). From the dried ethanol extract, 3 fractions were obtained after a liquid/liquid fractionation (hexane, ethyl acetate and water). Phytochemical screening of the raw extract was done using standard reactions. The phenol content was determined with Folin Denis reagent. Antioxidant activity was performed using 2, 2-diphenyl-picrylhydrazyl test (DPPH), ferric reducing antioxidant power (FRAP) and nitric oxide reducing tests. Results: Tannins, flavonoids and mucilage were the main phytochemical constituents of the leaf extract. Antioxidant assay revealed ethanol extract and its polar fractions were more active. In DPPH assay, the aqueous fraction (IC50: 61.93±5.99 μg / ml) was more active than the ethanol extract, the ethyl acetate and hexane fractions with respective IC50 values 74.1±4.47 - 81.53±6.51 - 1710.33±71.15 µg/ml. In nitric oxide test the ethyl acetate fraction (IC50: 56.66±3.09 µg/ml) had shown a better ability to inhibit nitric oxide radical than the ethanol extract, the hexane and aqueous fractions (IC50: 84.33±9.80- 451.33±1.24- 101.33±4.10 µg/ml respectively). In FRAP assay the ethyl acetate and aqueous fractions had shown a better activity than the leaf extract. Conclusion: Ethanol leaf extract had shown antioxidant activity in the different assays. This biological activity may be due to both polar and non polar compounds.
Cited by 9
Ndeye Adiara Ndiaye, Lahat Niang, Modou Dieng · Food and Nutrition Sciences · 2024
Galba Jean Beppe, Bertrand Mpoo Barga, Hervé Hervé Abaissou Ngatanko · Journal of Herbmed Pharmacology · 2024
Kang-Min Heo, Ji-Hyeon Park, Sam-pin Lee · Korean Journal of Food Preservation · 2018
Esse Adjoavi Agossou, Christine N’tcha, Gautier Roko · Journal of Biomedical Science and Engineering · 2026
Irene Alice Folefack, Sefirin Djiogue, Jean G. Beppe · International Journal of Research in Medical Sciences · 2025
Jounda Nadège Nelly, Nouga Bissoue Achille, Ndom Jean Claude · Journal of Pharmacognosy and Phytochemistry · 2023
Lahat Niang, Seid Ali Mahamat, Nicolas Cyrille Ayessou · Food and Nutrition Sciences · 2021
Ghislaine Boungou-Tsona, Maël Gainche, Caroline Decombat · Plants · 2023
Serigne Ibra Mbacke Dieng, Alioune Dior Fall, Kady Diatta-Badji · International Journal of Biological and Chemical Sciences · 2017
Related research
- In vitro Antioxidant and Anticancer Activity of Cyanobacteria — shares topic coverage
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- A Selective Review of Lipid Autoxidation and Rancidity, Antioxidants, and Dithiooxamide — shares topic coverage
- Antioxidant and Haemato-Biochemical Enhancing Properties of Daucus carota in Male Wistar Rats Toxicated with Lead Chloride — shares topic coverage
- Radical Scavenging Capacity and Efficacy of Myristica fragrans (Nutmeg) Metabolites on Cladosporum herbarum of Food Origin — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
9
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.