Biological Investigation of In-vitro Anti-Inflammatory, Antifungal, Anti-Arthritic, Thrombolytic, Membrane Stabilizing an In-vivo Acute Toxicological Activity of Launaea asplenifolia

Abstract

The present research is designed for evaluating the in-vitro activities of the ethanolic extract of Launaea asplenifolia (EELA), specifically its anti-inflammatory, antifungal, anti-arthritic, thrombolytic, membrane-stabilizing properties and acute toxicological activity. The present study aimed to conduct a primary evaluation of EELA through phytochemical screening. The screening revealed the presence of various secondary metabolites, including alkaloids, flavonoids, saponins, glycosides, carbohydrates, gums, and reducing sugars. These compounds are known to have diverse biological activities and are of interest to researchers in the fields of medicine. This research also investigated the anti-arthritic and anti-inflammatory therapeutic properties of EELA using in-vitro methods of protein denaturation. The results indicates that EELA had a significant impact on the reduction of arthritis and inflammation. The extracts demonstrated a percentage inhibition of 95.49% and 87.05% at a concentration of 1000 µg/mL, which were very significant compared to the standard diclofenac sodium (94.59%) and acetyl salicylic acid (88.88%) at the same concentration. This research investigated the potential antifungal properties of EELA using Disk Diffusion Method. The results of the research indicate that EELA exhibited antifungal activity against various types of fungi. The zone of inhibition ranged from 06-13 mm, depending on the specific type of fungi tested. The membrane-stabilizing properties of EELA using the heat-induced hemolysis method. The results indicated that EELA exhibited a protective effect against hemolysis, with a percent protection value of 82.02%. This value was compared to that of the standard diclofenac sodium, value of 73.63%. The thrombolytic activity of EELA was evaluated using the clot lysis method. The results indicated a positive correlation between the amount of extract used and the ability to break up blood clots. Notably, a value of 93.36% was obtained, which is statistically significant when compared to the standard streptokinase value of 91.304%. The in-vivo acute toxicological activity test was done using Cinnamon Oil Induced Method which showed 100% mortality rate at the dose of 5000mg/kg which was very potent compared to the cinnamon oil. In conclusion, the phytochemical present in the plant exhibits significant pharmacological effects, which may get used for drug discovery applications.