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Research Article Open access CC BY 4.0

Characterization of Antibiotic-producing Actinomycetes Isolated from River Tana and Lake Elementaita in Kenya

Bonface O. Shikuku, Silas Kiruki, Eric Kuria, Martin Mutembei, Fredrick O. Ogolla

Asian Journal of Research in Biochemistry · pp. 26–41 · Published 1 Aug 2023

10.9734/ajrb/2023/v13i1247

Abstract

The rise of antibiotic-resistant bacteria has become a global health concern, necessitating the search for novel sources of antibiotics. Actinomycetes, a group of microorganisms, have been known for their ability to produce bioactive compounds with antimicrobial properties. This study aimed to isolate, identify, and characterize antibiotic-producing Actinomycetes from River Tana and Lake Elementaita. Samples were collected from the study sites, and Actinomycetes were isolated using serial dilution and spread plate techniques. The isolates were characterized based on their morphological and biochemical properties. Furthermore, their antibacterial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhi was evaluated using the agar well diffusion method. The zones of inhibition were measured (mm), and analysis was done to compare the activity of the isolates using Kruskal Wallis test and medians compared using Wilcoxon with Bonferroni correction at alpha = 0.05 in SAS version 9.4. Analysis of DNA sequences was done using the BLAST program and a phylogenetic tree was constructed using MEGA X version 11. Biochemical tests revealed positive results for catalase, indole, oxidase, and citrate utilization, while coagulase and methyl red tests were negative. In terms of antibacterial activity, 54.5% of the isolates showed activity against E. coli, 45.5% against S. aureus, and 45.5% against S. typhi. Isolate LEL2201 had significant (p < 0.05) higher zone of inhibition against S. aureus (inhibition zone of 25.0mm), while isolate RT2201 exhibited the highest activity against E. coli and S. typhi (inhibition zone of 8.5 mm and 8.6 mm, respectively). Molecular characterization through 16S rRNA gene sequencing identified the isolates as belonging to the Actinobacterium order.                           Phylogenetic analysis revealed their similarity to known Actinomycetes species including Actinomycetales bacterium, Streptomyces intermedius and Streptomyces flavomacrosporus from various countries. The findings of this study demonstrate the presence of antibiotic-producing Actinomycetes in River Tana and Lake Elementaita. Thus, further investigations are warranted to identify and characterize the specific antibacterial compounds produced by these isolates.

References (28)

  1. 1 MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms [DOI]
  2. 2 Antimicrobial Photodynamic Therapy to Kill Gram-negative Bacteria [DOI]
  3. 3 Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia [DOI]
  4. 4 Plant-Microbe Interactions [DOI]
  5. 5 Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India) [DOI]
  6. 6 Streptomycetes: Characteristics and Their Antimicrobial Activities
  7. 7 Broad Spectrum Antimicrobial Activity of Forest-Derived Soil Actinomycete, Nocardia sp. PB-52 [DOI]
  8. 8 Molecular characterization and antibacterial effect of endophytic actinomycetes Nocardiopsis sp. GRG1 (KT235640) from brown algae against MDR strains of uropathogens [DOI]
  9. 9 An Improved Method for Isolation of Genomic DNA from Filamentous Actinomycetes
  10. 10 Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity [DOI]
  11. 11 Antibiotic producing microorganisms from River Wiwi, Lake Bosomtwe and the Gulf of Guinea at Doakor Sea Beach, Ghana [DOI]
  12. 12 ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES [DOI]
  13. 13 Isolation, Characterization, Screening and Antibiotic Sensitivity of Actinomycetes from Locally (Near MCAS) Collected Soil Samples [DOI]
  14. 14 Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India [DOI]
  15. 15 Species Diversity, Molecular Characterization, and Antimicrobial Susceptibility of Opportunistic Actinomycetes Isolated from Immunocompromised and Healthy Patients of Markazi Province of Iran [DOI]
  16. 16 Isolation and Identification of Actinomycetes Isoptericola variabilis From Cauvery River Soil Sample
  17. 17 Coagulase Test Protocol
  18. 18 Isolation, characterization and screening of actinomycetes from textile industry effluent for dye degradation
  19. 19 Integrated Profiling of Gram-Positive and Gram-Negative Probiotic Genomes, Proteomes and Metabolomes Revealed Small Molecules with Differential Growth Inhibition of Antimicrobial-Resistant Pathogens [DOI]
  20. 20 Effect of production parameters and inhibitory activity of antimicrobial compounds produced by co-cultured strains of Streptomyces xinghaiensis-OY62 and S. rimosus-OG95 [DOI]
  21. 21 Antimicrobial resistance [DOI]
  22. 22 Screening and Identification of Bacteria from Vellore Polycarbonate Lenses [DOI]
  23. 23 International Journal of Current Microbiology and Applied Sciences [DOI]
  24. 24 Research Journal of Pharmacy and Technology [DOI]
  25. 25 Journal of King Saud University – Science [DOI]
  26. 26 Recent Patents on Anti-Infective Drug Discovery [DOI]
  27. 27 international journal of engineering technology and management sciences [DOI]
  28. 28 Isolation of antibiotic producing microorganisms by screening for antibiotic resistance

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