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Research Article Open access CC BY 4.0

Chemical Identification, In vitro Antidiarrheal Potential, and Toxicological Study of the Leaves of Combretum grandiflorum G. Don (Combretaceae), a Beninese Medicinal Plant

Deguenon Memonsso Pierrette Pauline, Alban Gouton HOUNGBEME, Ahoyo Carlos Cédric, Ombouma Joanna Grâce, Gbaguidi Ahokanou Fernand, Houinato Marcel Romuald Benjamin

International Journal of Plant & Soil Science · pp. 954–977 · Published 13 Dec 2023

10.9734/ijpss/2023/v35i224207

Abstract

Combretum grandiflorum G. Don is traditionally used in ethnic medicines for the treatment of different diseases in Benin. Despite its wide use, no studies have been undertaken on its potential toxicity. The study was designed to assess the antimicrobial activity and potential toxicity of aqueous and hydroethanolic extracts of C. grandiflorum. Qualitative phytochemistry was carried out by a differential staining or precipitation method. The antibacterial and antifungal effects were evaluated by the solid medium diffusion method. Cytotoxicity was evaluated on Artemia salina larvae while acute toxicity was evaluated with Wistar strain rats via the oral route. The qualitative screening revealed the presence in the plants of Polyphenols, Flavonoids, Tannins, catechic and gallic tannins. Both types of extracts inhibited microbial growth. The hydroethanolic extract was active on all the bacterial strains used with a high inhibition diameter compared to the aqueous extract. On strains of Shigella Sonnei and Salmonella spp, the antibacterial effect of the hydroethanolic extract is significantly (p˂0.05) better compared to that of the aqueous extract. Cytotoxicity tests showed no toxic effects at the doses evaluated. At a dose of 2000 mg/kg, the aqueous and hydroethanolic extracts of C. grandiflorum induced neither mortality nor alteration of the physiological parameters of Wistar rats (renal, hepatic parameters, hematological constants, body weight). These extracts therefore do not present acute oral toxicity for Wistar rats at the limit dose of 2000 mg/Kg/bw in Wistar rats. These results justify some therapeutic indications for these plants. They could therefore be used for the development of improved phytomedicines.

Combretum grandiflorum Benin antimicrobial activity Cytotoxicity acute toxicity

References (31)

  1. 1 Modes of Action of Herbal Medicines and Plant Secondary Metabolites [DOI]
  2. 2 Ethanolic Extract of Propolis (EEP) Enhances the Apoptosis- Inducing Potential of TRAIL in Cancer Cells [DOI]
  3. 3 Herbal versus synthetic drugs; beliefs and facts.
  4. 4 Brazilian Journal of Pharmaceutical Sciences
  5. 5 Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents [DOI]
  6. 6 Herbal Medicines in African Traditional Medicine [DOI]
  7. 7 Childhood diarrhoeal diseases in developing countries [DOI]
  8. 8 Acute oral toxicity evaluation of aqueous ethanolic extract of Saccharum munja Roxb. roots in albino mice as per OECD 425 TG [DOI]
  9. 9 Bioactivities of the Genus Combretum (Combretaceae): A Review [DOI]
  10. 10 Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded Opuntia stricta fruit [DOI]
  11. 11 Evaluation of acute and sub-acute toxicity of selected traditional antiurolithiatic medicinal plant extracts in Wistar albino rats [DOI]
  12. 12 Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria [DOI]
  13. 13 A pharmacology‐based comprehensive review on medicinal plants and phytoactive constituents possibly effective in the management of COVID ‐19 [DOI]
  14. 14 Evaluation of antioxidant and antibacterial activities of methanolic extract of medlar (Mespilus germanica L.) leaves [DOI]
  15. 15 Natural and Synthetic Drugs as Potential Treatment for Coronavirus Disease 2019 (COVID-2019) [DOI]
  16. 16 Toxicological Characterization of Ten Medicinal Plants of the Beninese Flora Used in the Traditional Treatment of Diarrheal Diseases [DOI]
  17. 17 Treatment of Diarrhoea in Rural African Communities: An Overview of Measures to Maximise the Medicinal Potentials of Indigenous Plants [DOI]
  18. 18 Diuretic and antiurolithiatic activities of an ethanolic extract of Acorus calamus L. rhizome in experimental animal models [DOI]
  19. 19 Antibacterial, antifungal and antioxidant activities of whole plant chemical constituents of Rumex abyssinicus [DOI]
  20. 20 Toxicological studies on the aqueous leaf extract of Pavetta crassipes (K. Schum) in rodents [DOI]
  21. 21 Structural elucidation of phenolic compounds isolated from Opuntia littoralis and their antidiabetic, antimicrobial and cytotoxic activity [DOI]
  22. 22 Bioactivity of Medicinal Plants and Extracts [DOI]
  23. 23 Garcinia kola (Heckel) and Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. from Cameroon possess potential antisalmonellal and antioxidant properties [DOI]
  24. 24 Exploration of the Chemical Potential and Antioxidant Activity of Some Plants Used in the Treatment of Male Infertility in Southern Benin [DOI]
  25. 25 Possible Antioxidant and Antidiabetic Effects of Combretum Molle Extract in a Diabetes Mellitus Experimental Model in Male Rats [DOI]
  26. 26 Antifungal effect of sesame medicinal herb on Candida Species: original study and mini-review [DOI]
  27. 27 Chemical Composition and Biological Properties of the Petroleum Ether Extract of Solanum macrocarpum L. (Local Name: Gorongo) [DOI]
  28. 28 Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants [DOI]
  29. 29 Antidiarrheal Activity of Dialium guineense Willd Fruit Pulp in Wistar Rats [DOI]
  30. 30 Antibiotics Resistance Profile and Antibacterial Activities of Combretum micranthum and Combretum adenogonium Extracts on Clinical Isolated Vibrio cholera [DOI]
  31. 31 Indiscriminate Use of Antibiotics for COVID-19 Treatment in South Asian Countries is a Threat for Future Pandemics Due to Antibiotic Resistance [DOI]

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