Comparative Study of Estradiol-Supported In-vitro Maturation in Hariana and Sahiwal Oocytes under Weekly and Bi-Weekly Protocols
Anoop Kumar, Atul Saxena, Mukul Anand, Ashutosh Mishra, Amrita Priyadershi, Pratyanshu Srivastava
Journal of Advances in Biology & Biotechnology · pp. 816–822 · Published 18 Dec 2025
10.9734/jabb/2025/v28i123429Abstract
Estradiol is a key hormone that helps in the development and growth of bovineoocytes, playing an important role in in-vitro maturation (IVM) outcomes for cattle. This study evaluated the effects of estradiol supplementation at concentrations of 0.5 μg/mL, 1.0 μg/mL, and 1.5 μg/mL on the maturation of oocytes retrieved from super-stimulated Hariana and Sahiwal cows (n = 6 per breed) using ovum pick-up (OPU) protocols. Weekly and bi-weekly OPU intervals were compared to assess the influence of retrieval frequency. Maturation rates were evaluated through cumulus expansion and polar body extrusion. Data were analyzed using one-way ANOVA with significance set at 5% and 1%. The results revealed optimal maturation rates of 80.44% at 1.5 μg/mL and 73.88% at 1.0 μg/mL estradiol, respectively, with weekly OPU yielding superior outcomes. These findings provide valuable insights into refining IVM protocols and advancing assisted reproductive technologies (ARTs) for sustainable cattle breeding.
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