Genetic Kinship of Nilem Fish Strains  (Osteochillus hasselti)

Yuli Andriani; Ibnu Dwi Buwono; Ujang Subhan; Arini Mandhasia

doi:10.9734/ajbgmb/2020/v3i130077

Research Article Open access CC BY 4.0

Genetic Kinship of Nilem Fish Strains (Osteochillus hasselti)

Yuli Andriani, Ibnu Dwi Buwono, Ujang Subhan, Arini Mandhasia

Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 28–37 · Published 14 Jan 2020

10.9734/ajbgmb/2020/v3i130077

Abstract

The research aims to analyze the kinship of nilem fish strains using RAPD (Random Amplified Polymorphic DNA). This research used exploratory method with descriptive analysis. The primers used in the RAPD method are OPA-02 (5'-TGCCGAGCTG-3 '), OPA-03 (5'-AGTCAGCCAC-'3), OPA-05 (5'-AGGGGTCTTG-3') and OPA-11 (5) 5'- CAATCGCCGT-3 '). The primary optimization results showed that OPA-02 and OPA-11 were the primary primers for detecting polymorphic and monomorphic fragments of the nilem fish genome. Phenograms from the RAPD method that were processed through the NTSYS-pc program showed that the OPA-02 primer was the best primer for nilem kinship analysis. Relationship between red nilem with green nilem is 92%, red nilem and green nilem with mangot nilem are 72% and the three nilem with beureum panon are 12%.

Kinship RAPD nilem strain.

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Yuli Andriani, Ibnu Dwi Buwono, Ujang Subhan, Arini Mandhasia (2020). Genetic Kinship of Nilem Fish Strains (Osteochillus hasselti). Asian Journal of Biochemistry, Genetics and Molecular Biology , 28–37. https://doi.org/10.9734/ajbgmb/2020/v3i130077

Affiliations

Yuli Andriani — Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Ibnu Dwi Buwono — Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Ujang Subhan — Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Arini Mandhasia — Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.

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