Detection of INVA Gene and Cytotoxin of Salmonella entertidis in Food Samples Using Molecular Methods
Maha Almas, Usaal Tahir, Mariam Zameer, Muhammad Imtiaz Shafiq, Syeda Yumna Farrukh, Nureen Zahra, Maham Mazhar, Sajjad Ali Gill, Faheem Hadi, Tahir Muhammad, Qurban Ali, Arif Malik
Journal of Pharmaceutical Research International · pp. 20–28 · Published 16 Mar 2021
10.9734/jpri/2021/v33i1231249Abstract
Salmonella entertidis is a foodborne pathogen that causes various diseases in human beings worldwide. The toxin of Salmonella can cause infectious diseases. In this research project, Salmonella was detected through various microbial, biochemical and molecular tests in diverse food samples collected from highly populated, moderately populated and less populated areas of Lahore, Pakistan. Enriched cultures of all food samples such as apples, tomatoes, yogurt and mayonnaise was streaked on violet-red bile glucose agar, Simmon’s citrate agar and eosin-methylene blue agar (EMB). Salmonella isolates were screened for the presence of toxin encoding gene through PCR. 27% apples, 19% tomatoes, 5% mayonnaise and 7% yogurt were found to be positive for INVA genes (invasion protein genes). In medical and pharmaceutical point of views the INVA gene can also help to develop specific medicines against salmonella. The cytotoxin that is protein in nature was confirmed by SDS PAGE in mayonnaise samples. This study illustrates that foods of highly populated areas are reservoir for Salmonella entertidis in Pakistan. There is need to develop specific drugs, precautionary measures to control salmonella and its disease.
Cited by 6
A P Wijaya, A Sabdono, M T Sibero · IOP Conference Series: Earth and Environmental Science · 2022
Mingyan Chai · International Journal of Electrochemical Science · 2022
ALDI PRATAMA WIJAYA, AGUS SABDONO, MADA TRIANDALA SIBERO · Biodiversitas Journal of Biological Diversity · 2022
Reem Zuhair Shaker, Atheer Abdel Razzaq Abdel Aziz, Ruqaya Mustafa Ali · AIP Conference Proceedings · 2023
Asmaa G. Mubarak, Mona M. Mustafa, Mohamed W. Abdel-Azeem · Advances in Animal and Veterinary Sciences · 2021
M.M. Fathuddin, S.A. Ado, M.B. Tijjani · Nigerian Journal of Biotechnology · 2024
Related research
- Occupational Health Regulations in Nigeria: A Narrative Overview — shares topic coverage
- Review on Emerging and Reemerging Infectious Diseases and Their Origins — shares topic coverage
- Assessment of Microbial Contaminations Associated with Steering Wheels and Palms of Commercial Drivers at the University of Cape Coast’s Taxi Rank — shares topic coverage
- Bioactive Compounds in Fish and Their Health Benefits — shares topic coverage
- Food Derived Bioactive Peptides for Health Enhancement and Management of Some Chronic Diseases — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
6
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.