Skip to content
Research Article Open access CC BY 4.0

Determination of Anti-oxidant Activity of Ethanol, Ethyl acetate, Hexane, and Aqueous Extracts of Sri Lankan Traditional Poly-herbal Formula Nāgarādi Panchakaya

Waratenne P. R., Jayasiri A. P. A., Manuha I. M.

Asian Journal of Research in Biochemistry · pp. 1–8 · Published 8 Nov 2023

10.9734/ajrb/2023/v13i4266

Abstract

Natural products are the good source of natural antioxidants. Nāgarādi panchakaya is a well-known poly-herbal formula prescribed specially for upper respiratory tract diseases by Sri Lankan Ayurveda medical practitioners. The name implies that it contains five herbal ingredients including Inguru (Zingiber officinale), Devadāra (Cedrus deodara), Kottammalli (Coriandrum sativum), Ela batu (Solanum indicum) and Katuwelbatu (Solanum xanthocarpum). This research study was aimed to determine the antioxidant activity of Nāgarādi panchakaya, extracted with different solvents (ethanol, ethyl acetate, n-hexane and water). Antioxidant activity was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay method and the Ferric Reducing Antioxidant Power (FRAP) assay. In vitro antioxidant free radicals scavenging capacities of different concentrations of ethanol, ethyl acetate, n-hexane and water extracts, of Nāgarādi panchakaya were evaluated. The percentage of inhibition and IC50 were calculated. IC50 (μg/mL) of water, ethanol, ethyl acetate, n-hexane and Ascorbic Acid were found to be 179.89±1.25 µg /mL, 248.09±1.74 µg /mL, 169.43± 0.98µg /mL, 189.56±2.23 µg /mL, and 01.18±0.98 µg /mL respectively in DPPH radical scavenging assay. In FRAP assay the reducing power for the different extracts varied in following order: water > ethanol > ethyl acetate > n-hexane. The total Phenolic content of water, ethanol, ethyl acetate and n-hexane extracts were 1.73±0.51 mg GAE/g, 2.59±0.67 mg GAE/g, 3.67±0.81 mg GAE/g and 1.57±0.09 mg GAE/g respectively. The results can be concluded that Ethanol & water extracts exhibit high antioxidant activity possibly due to the presence of high phenolic content than the other extracts.

Antioxidant DPPH FRAP Nāgarādi panchakaya

References (14)

  1. 1 Antioxidant Activity and Phenolic Compounds in Selected Herbs [DOI]
  2. 2 Antioxidant activity and phenolic compounds in 32 selected herbs [DOI]
  3. 3 In vitro antioxidant activity of Anthriscus cerefolium L. (Hoffm.) extracts [DOI]
  4. 4 Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea [DOI]
  5. 5 Antioxidant therapies in COPD [DOI]
  6. 6 Antioxidant Capacity Connection with Phenolic and Flavonoid Content in Chinese Medicinal Herbs [DOI]
  7. 7 Antioxidant activity, phenolic compound contents and antimutagenic activity of some water extract of herbs [DOI]
  8. 8 Study of antioxidant activity and some herbal compounds of Dracocephalum kotschyi Boiss. in different ages of growth [DOI]
  9. 9 New Insights into the Role of Glutathione in the Mechanism of Fever [DOI]
  10. 10 Overview of the immune response [DOI]
  11. 11 Dietary Antioxidants and Infectious Diseases [DOI]
  12. 12 Infectious Diseases and Your Health [DOI]
  13. 13 Phytochemical Analysis of Different Extracts of Nagaradi panchakaya
  14. 14 The Journal of Allergy and Clinical Immunology [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

26

Page views

0

PDF downloads

14

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.