In silico and in vivo Biological Evaluation of Chenopodium quinoa on Scopolamine Induced Memory Impairment in Mice

Abstract

Aims: The present research is focused on screening in vivo and insilico anti- amnesic activity of the ethanolic extract of seeds of Chenopodium quinoa. To establish the exact the mechanism of action, the phytochemical constituents of the extract were subjected to in silico studies using schrodinger and pass software. Methodology: The powdered material of seeds of Chenopodium quinoa were dried and extracted with ethanol by soxhlation technique. In vivo evaluation of anti- amnesic activity of the ethanolic extract of seeds of Chenopodium quinoa was carried using Transfer latency by Elevated plus maze, Novel object recognition test and Passive avoidance time by cook’s pole climbing apparatus. The major phytoconstituents were subjected to molecular docking using PDB ID: 1EVE, 6N33, 2FV5 and 6W2X. Prediction studies for biological activities, adverse effects, direct and possible targets and using PASS (Predictions of activity spectra for substances) online software. Results: The extract was administered at doses (200 mg/kg and 400 mg/kg) showed significant anti- amnesic activity. The prolongation of the transfer latency time (secs) in the elevated plus-maze test is as an indicator for impairment of learning and memory. In Novel objective recognition test the time of memorizing the familiar object with that of the novel object and time taken for each animal to recognize the novel object compare to familiar object is noted. The results revealed that Quercetin, Beta sitosterol, Kaempferol, Dimethyl sulphide, Myristic acid, Palmitic acid, Stigmasterol, Lenolenic acid, Pentadecanoic acid, Tocopherols, Arachidonic acid and standard Donepezil have got highest glide scores against PDB ID: 1EVE, 6N33, 2FV5 and 6W2X. The ADME results revealed the higher oral bioavailability. The major phytoconstituents were subjected to prediction studies for biological activities, adverse effects, direct and possible targets and using PASS (Predictions of activity spectra for substances) online software. Conclusion: From in vivo and in silico results it is evident that ethanolic extract seeds of Chenopodium quinoa possessed significant anti- amnesic activity.