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Research Article Open access CC BY 4.0

Ecology and Protein Composition of Polypedates leucomystax (Gravenhorst, 1829) (Anura: Rhacophoridae) Foam Nests from Peninsular Malaysia

Shahriza Shahrudin, Mohd Nazri Ismail, Soon Hong Kwan, Nazalan Najimudin

Annual Research & Review in Biology · pp. 1–10 · Published 18 Jul 2017

10.9734/ARRB/2017/34211

Abstract

The four-lined tree frog, Polypedates leucomystax, spawns its eggs in a moist structure called a foam nest. Four foam nests constructed by this species were collected from the Sungai Sedim Recreational Forest, Kedah, Peninsular Malaysia. Two foam nests were found deposited on the leaves of low vegetation hanging over a rock pool. One was attached inside a water tank, and one was found on grass near an ephemeral puddle. In the laboratory, the foam nests were freeze-dried and the protein concentrations quantified, fractionated, and analyzed using LC-MS/MS. Twenty-two proteins, including seven enzymes, six structural proteins, five regulatory proteins, three receptors, and one antimicrobial peptide (AMP) were found in the foam nests. The function of the AMP (brevinin-2 type) is believed to protect the frog eggs from pathogenic microorganisms.

Amphibian foam nest protein and peptide pharmacological effect Polypedates leucomystax Peninsular Malaysia.

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Cited by 23

Form, function, foam: evolutionary ecology of anuran nests and nesting behaviour

Eva K. Fischer · Philosophical Transactions of the Royal Society of London. Biological Sciences · 2023

Pioneer access of the foam nest bacterial community of Leptodactylidae frogs and its biotechnological potential.

L. G. Z. Castro, M. Sousa, L. É. C. Pereira · Brazilian journal of biology = Revista brasleira de biologia · 2024

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