Effect of Different Media, Temperature, pH and Nitrogen Sources on Growth and Development of Helminthosporium oryzae Causing Brown Leaf  Spot of Rice

Gaurav Ayodhya Singh; Ramji Singh; Kamal Khilari; Gopal Singh; D.V. Singh; Uday Pratap Shahi

doi:10.9734/ijpss/2024/v36i84879

Research Article Open access CC BY 4.0

Effect of Different Media, Temperature, pH and Nitrogen Sources on Growth and Development of Helminthosporium oryzae Causing Brown Leaf Spot of Rice

Gaurav Ayodhya Singh, Ramji Singh, Kamal Khilari, Gopal Singh, D.V. Singh, Uday Pratap Shahi

International Journal of Plant & Soil Science · pp. 486–493 · Published 3 Aug 2024

10.9734/ijpss/2024/v36i84879

Abstract

The pathogen Helminthosporium oryzae was subjected to different cultural conditions viz., media, temperature, pH and nitrogen sources under in vitro conditions. The supreme radial progress of Czapeck Agar, OMA & PDA was recorded highest with mean radial growth followed by Richard’s agar (88 mm), Water agar. The colony showed up as thick, leathery, slightly elevated, and abundant mycelia with brown-coloured conidia on this medium. Its colour ranged from greyish-white to dark brown. When exposed to constant light, the average mycelial growth of the fungus was measured at 58.60 mm, 51.41 mm at alternating light cycles, and 72.80 mm at total darkness. When the pathogen was assessed at various pH levels, the fungus's maximum radial growth was observed at pH 7.0 (90.00), and its least radial growth was observed at pH 4.5 (44.89 mm) and pH 5.0 (60.33 mm). Ammonium chloride showed the least growth among the various nitrogen sources, while potassium nitrate showed the greatest development.

Radial growth media temperature nitrogen pH

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Gaurav Ayodhya Singh, Ramji Singh, Kamal Khilari, Gopal Singh, D.V. Singh, Uday Pratap Shahi (2024). Effect of Different Media, Temperature, pH and Nitrogen Sources on Growth and Development of Helminthosporium oryzae Causing Brown Leaf Spot of Rice. International Journal of Plant & Soil Science , 486–493. https://doi.org/10.9734/ijpss/2024/v36i84879

Affiliations

Gaurav Ayodhya Singh — Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh, India.
Ramji Singh — Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh, India.
Kamal Khilari — Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh, India.
Gopal Singh — Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh, India.
D.V. Singh — Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut- 250110, Uttar Pradesh, India.
Uday Pratap Shahi — Department of Soil Science, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh, India.

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