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Research Article Open access CC BY 4.0

Effect of Nano Zinc Oxide Supplementation on Blood Parameters of Crossbred Calves

Ajay Barman, Lakhyajyoti Borah, Robin Bhuyan, B. N. Saikia, Reema Saikia, Meenakshi Kalita

Journal of Advances in Biology & Biotechnology · pp. 416–421 · Published 10 Feb 2026

10.9734/jabb/2026/v29i23649

Abstract

The goal of the current study was to assess how reinforcing crossbred calves with inorganic and nano forms of zinc affected their growth and overall health. A completely randomized design (CRD) was used to divide 24 crossbred (Jersey × Holstein Friesian) of 6-9 months age into three (3) homogeneous groups based on their sex and live body weight. Animals were divided into three groups containing 8 number of animals in each group- T1, T2 and T3. The T1, T2, and T3 groups, which received supplements containing inorganic ZnO @ 40mg/kg DM, Nano ZnO@ 10mg/kg DM and Nano ZnO @ 20mg/kg DM, respectively, in addition to the basic diet. Blood biochemical parameters i.e. serum cholesterol, total triglycerides, serum creatinine, serum GGT, serum albumin/globulin ration and super oxide dismutase were estimated at the 0 day (initial), 42nd day (mid) and 84th day (end) of the feeding trial. Statistically no significant difference was observed among the groups in respect of the total cholesterol, serum triglycerides, Serum creatinine, Serum gamma glutamyl transference, and albumin: globulin values. Significant difference was observed among the groups in 42nd and 84th days of experiment regarding SOD level.

Cross bred calves nano zinc oxide immunity

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