Effect of Aqueous Extract of Rauvolfia vomitoria (Apocynaceae) Leaves with on Sexual Activity of Male Rats

Abstract

Objective: The aim of this study is to determine the pharmacological effects of Rauvolfia vomitoria leaves on the sexual activity of animal model (male rat) Methods: Acute toxicity was performed according to OECD Guideline 423, by a dose limit of 2000 mg /kg body weight. The observations (during 14 days) focused on the number of deaths, convulsions, sleep and coma. Study of sexual activity has been realized by using 20 male rats, distributed in 4 lots of 5 animals. Group 1 treated with distilled water, group 2 treated with 5 mg/kg B.W. of sildenafil citrate and the other two batches received respectively the doses 500 and 1000 mg/kg of the aqueous extract of Rauvolfia vomitoria. Females in estrus state (in heat) were introduced for a period of 30 minutes. During this period, parameters of sexual behavior were recorded. At the end of the 8-day treatment, organs such as penis testicles, seminal vesicles, prostate, epididymis and elevator muscle were removed. These organs were studied in order to determine pharmacological effects of aqueous extract of Rauvolfia vomitoria. Results: The aqueous extract of Rauvolfia vomitoria showed no evidence of single dose toxicity (2000 mg/kg) when studying acute toxicity. Ride latency time, coitus latency time and ejaculation latency time was significantly decreased (P<0.001) with regard to negative control (distilled water). Ride frequency, coitus fraquency and ejaculation frequency was significantly increased (P<0.001) with regard to negative control (distilled water). No significant difference (P˃0.05) has been recorded on organ androgeno-dependant. Conclusion: Aqueous extract of R. vomitoria has sexual stimulating activity or an aphrodisiac potential which could justify its traditional use. The aphrodisiac potential is higher at the dose of 1000 mg/kg b.w .