Prevalence and Phenotypic Characterization of Extended-Spectrum β- Lactamase (ESBL)-Producing Uropathogenic Enterobacteriaceae Isolated at the Fann University Hospital

Abstract

Introduction: Urinary tract infections caused by extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-producing Enterobacteriaceae) are a public health problem in the world. This study aims to determine the prevalence and resistance profiles of ESBL-producing Enterobacteriaceae isolated at the Fann University Hospital Center. Methods: This is a prospective study of urine samples analyzed at the bacteriology laboratory of the Fann University Hospital Center. Bacterial identification and antibiograms were performed according to CA-SFM recommendations. During the study, 2,852 urine samples were collected from patients who came for consultation or were hospitalized in the study facility. Results: Of all the enterobacteria isolated from 20% of urine samples, 32% were ESBL-producing. ESBL-producing E. coli were mainly isolated from outpatients (71%) and elderly subjects (≥ 60 years: 44%). The main co-resistances concerned gentamicin (66%), cotrimoxazole (88%), and fluoroquinolones (75%). Good sensitivity to imipenem (80%) and chloramphenicol (80%) was observed. Conclusion: These results confirm the high community prevalence of ESBL-producing E. coli in Dakar and the threat of multidrug resistance. The continued sensitivity to carbapenems should encourage ongoing surveillance and prudent use of antibiotics in Senegal.