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Research Article Open access CC BY 4.0

Evaluation of Cefoxitin Disc Diffusion and Chromogenic Agar in the Detection of Methicillin Resistant Staphylococcus aureus

A. I. Umar, I. Garba, A. M. Ganau

South Asian Journal of Research in Microbiology · pp. 20–26 · Published 20 Jan 2023

10.9734/sajrm/2023/v15i1278

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) has a worldwide distribution and is an important cause of clinical and epidemiological problems. The aim of this study was to evaluate the usefulness of some phenotypic methods for the detection of methicillin resistant S. aureus in clinical laboratories. The study is cross sectional. A total of 93 S. aureus isolates were tested using cefoxitin disk diffusion (CDD) and oxacillin resistance screening agar base (ORSAB) with reference to mecA gene PCR. Of the 93 isolates, CDD test showed 34 were MRSA, while ORSAB recorded 42. MecA gene was detected by PCR in 34 of the isolates. The CDD showed 97.1% sensitivity and 98.3% specificity and therefore superior to ORSAB with sensitivity 97.1% and specificity 84.7%. The cefoxitin disk test required no special test conditions and can improve the reliability of routine tests for the detection of MRSA. CDD test can thus be used as a cheap and reliable alternative to PCR for the detection of MRSA in resources limited settings.

Cefoxitin disk Chromogenic agar mecA MRSA Staphylococcus aureus

References (20)

  1. 1 Staphylococcus aureus Infections [DOI]
  2. 2 Clinical Microbiology Procedures Handbook [DOI]
  3. 3 Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction [DOI]
  4. 4 Microbial Diversity in Water and Sediment of Lake Chaka, an Athalassohaline Lake in Northwestern China [DOI]
  5. 5 Mobile Genetic Element-Encoded Cytolysin Connects Virulence to Methicillin Resistance in MRSA [DOI]
  6. 6 COMPARISON OF CEFOXITIN DISC DIFFUSION TEST, OXACILLIN SCREEN AGAR, AND PCR FOR mecA GENE FOR DETECTION OF MRSA [DOI]
  7. 7 Clinical microbiology procedures handbook
  8. 8 Clinical and economic impact of methicillin resistance in patients with Staphylococcus aureus bacteremia [DOI]
  9. 9 Clinical and Economic Analysis of Methicillin-Susceptible and -Resistant Staphylococcus aureus Infections [DOI]
  10. 10 Methicillin-resistant Staphylococcus aureus on orthopaedic wards [DOI]
  11. 11 Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus [DOI]
  12. 12 Evaluation and comparison of tests to detect methicillin resistant S. aureus [DOI]
  13. 13 Calculations for molecular biology and biotechnology : a guide to mathematics in the laboratory
  14. 14 Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha [DOI]
  15. 15 Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran
  16. 16 Evaluation of Brilliance MRSA 2 Agar for Detection of Methicillin-Resistant Staphylococcus aureus in Clinical Samples [DOI]
  17. 17 Comparison of Real-Time PCR with Disk Diffusion, Agar Screen and E-test Methods for Detection of Methicillin-Resistant Staphylococcus aureus [DOI]
  18. 18 Evaluation of a New Medium, Oxacillin Resistance Screening Agar Base, for the Detection of Methicillin-Resistant Staphylococcus aureus from Clinical Specimens [DOI]
  19. 19 Comparison of Cefoxitin Disk Diffusion Test, Automated System and Chromogenic Medium for Detection of Methicillin Resistance in Staphylococcus aureus Isolates [DOI]
  20. 20 DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) BY AUTOMATED AND MANUAL METHODS

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