Development of Sequence Characterized Amplified Region (SCAR) Marker for the Authentication of Bacopa monnieri (L.) Wettst
Aradhana Yadav, Javed Ahmad, Anis A. Chaudhary, Altaf Ahmad
European Journal of Medicinal Plants · pp. 186–198 · Published 3 Apr 2012
10.9734/EJMP/2012/1192Abstract
Aims: To develop Sequence Characterized Amplified Region (SCAR) marker for identification of Bacopa monnieri (L.) Wettst. Study design: Molecular biology tools for authentic identification of Bacopa monnieri. Methodology: RAPD-based SCAR marker was developed to identify Bacopa monnieri from its adulterant candidates namely Centella asiatica, Eclipta alba and Malva rotundifolia. 50 random primers were used for initial screening of different accessions of Bacopa monnieri, Eclipta alba and Malva rotundifolia. A putative 589 bp marker specific to Bacopa monnieri was identified using RAPD technique. This RAPD-amplicon was then sequenced and cloned. Based on the information of cloned sequences a pair of SCAR primers was designed. SCAR primers were then used for authentication of DNA samples of Bacopa monnieri and its adulterants. Market samples of Bacopa monnieri and Centella asiatica collected under the name of Brahmi was put to test with these primers. Results: Out of 50 random primers, only 14 primers were able to amplify the above plants. A 589 bp polymorphic band obtained with OPAA-3 primer which was specific to Bacopa monnieri accessions and not found in other adulterant candidates was selected. This band was eluted, cloned and further sequenced. A pair of SCAR primers (Bac F & Bac R) between 406 bp of 589 bp sequence of RAPD amplicon was designed. A single, bright, distinct band was obtained in Bacopa monnieri and not in the adulterants. Further validation was also done in the market samples. Conclusion: In essence, the study was to develop a RAPD-based SCAR marker for authentication of Bacopa monnieri. The SCAR marker was found to be useful for preventing the adulteration of other plants in Brahmi and also for screening of crude drug samples intended for export and domestic uses.
Cited by 18
Jill M. Hoffman, Jean-Louis Lafeuille, Subramanyam Ragupathy · Food Fraud · 2021
Mohammad Ajmal Ali · Saudi Journal of Biological Sciences · 2019
Rita Biltes, Caterina Villa, Joana Costa · Foods · 2025
Ramanujam Srirama, J. U. Santhosh Kumar, G. S. Seethapathy · Drug Safety · 2017
Showkat Hussain Ganie, Priti Upadhyay, Sandip Das · Plant Gene · 2015
D. B. Anantha Narayana, Sudhakar T. Johnson · Plant Biotechnology Reports · 2019
Abhi P. Shah, Tasnim Travadi, Sonal Sharma · 2022
Abhi P. Shah, Tasnim Travadi, Sonal Sharma · Molecular Biology Reports · 2023
Jean-Marie Botto, Loïc Loffredo, Gopinathan K. Menon · Cosmetics · 2025
Naseer Mohammad, Ankur Dahayat, Harshita Agrahari · Biotechnological Approaches for Sustaining Forest Trees and Their Products · 2024
Related research
- Prevalence of Adulteration and Inhibitory Substances in Raw and Pasteurized Milk Marketed in Nairobi Region — shares topic coverage
- Physicochemical Evaluation of Bovine Milk in North Central Nigeria — shares topic coverage
- Exploring Margarine in Anhydrous Milk Fat by Chromatographic Tools — shares topic coverage
- Microscopical Characters, Heavy Metals Level and Histopathological Effects of Lawsonia inermis L. Leaves in Female Mice — shares topic coverage
- Microbial Features and Qualitative Detection of Adulteration along with Physicochemical Characteristics of Sweetened Yoghurt — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
18
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.