Studies on Bio-color Production by  Pseudomonas aeruginosa Isolated from Soil

M. U. Hizbullah; A. A. Farouq; A. S. Baki; M. U. Dabai; A. Nafi’u; M. K. Nata’ala; G. Mustapha

doi:10.9734/JAMB/2018/43068

Research Article Open access CC BY 4.0

Studies on Bio-color Production by Pseudomonas aeruginosa Isolated from Soil

M. U. Hizbullah, A. A. Farouq, A. S. Baki, M. U. Dabai, A. Nafi’u, M. K. Nata’ala, G. Mustapha

Journal of Advances in Microbiology · pp. 1–12 · Published 10 Sep 2018

10.9734/JAMB/2018/43068

Abstract

This study aimed at producing biocolor from soil inhabiting bacteria. Soil samples were screened for isolation of green pigment-producing bacteria and identified molecularly using the standard method. The effect of medium, pH, temperature, incubation time, shaking and static conditions on color production were determined on the isolate, and the pigment was extracted by using chloroform. It was observed that green pigment was produced by Pseudomonas aeruginosa in Nutrient broth at pH 7, after 72 hours incubation and at the temperature of 37°C under shaking condition at 4,000 rpm for 15 minutes. The pigments were characterized and identified as pyocyanin using Thin Layer Chromotography (TLC), Fourier Transformed Infrared Spectroscopy (FTIR) and UV-Visible spectroscopy (UV). The stability of the pigment was tested based on pH and temperature. It was found that the green pigment showed stability at 160°C, 200°C and pH 13.

Pyocyanin TLC Pseudomonas aeruginosa FTIR and green-pigment

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M. U. Hizbullah, A. A. Farouq, A. S. Baki, M. U. Dabai, A. Nafi’u, M. K. Nata’ala, G. Mustapha (2018). Studies on Bio-color Production by Pseudomonas aeruginosa Isolated from Soil. Journal of Advances in Microbiology , 1–12. https://doi.org/10.9734/JAMB/2018/43068

Affiliations

M. U. Hizbullah — Department of Microbiology, Sokoto State University, Along Birnin Kebbi Road Sokoto State, Nigeria
A. A. Farouq — Department of Microbiology Faculty of Science Usmanu Danfodiyo University, Sokoto, Nigeria
A. S. Baki — Department of Microbiology Faculty of Science Usmanu Danfodiyo University, Sokoto, Nigeria
M. U. Dabai — Department of Chemistry, Faculty of Science, Usmanu Danfodiyo University, Sokoto, Nigeria
A. Nafi’u — Department of Microbiology, Sokoto State University, Along Birnin Kebbi Road Sokoto State, Nigeria
M. K. Nata’ala — Department of Microbiology Faculty of Science Usmanu Danfodiyo University, Sokoto, Nigeria
G. Mustapha — Department of Microbiology, Sokoto State University, Along Birnin Kebbi Road Sokoto State, Nigeria

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